Gene expression and evolution of Family-1 UDP-glycosyltransferases-insights from an aquatic flowering plant (sacred lotus)
Gene expression and evolution of Family-1 UDP-glycosyltransferases-insights from an aquatic flowering plant (sacred lotus)
复制标题
Family-1 UDP-糖基转移酶的基因表达和进化——来自水生开花植物（圣莲）的见解
DOI：
10.1016/j.aquabot.2020.103270
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发表时间：
2020-08-01
期刊：
影响因子：
1.8
通讯作者：
Shi, Tao
中科院分区：
文献类型：
--
作者：
Li, Hui;Yang, Xingyu;Shi, Tao
家族-1 UDP-糖基转移酶，称为UGT，将关键的细胞代谢物糖基化为具有改善的稳定性、溶解性和运输的化合物，以调节植物在各种代谢途径中的生长和发育。然而，关于水生植物中UGT家族的分子和功能特征以及其在莲生态分化中的作用还知之甚少。因此，我们进行了全基因组调查的108个候选UGT基因分布在所有8条染色体上，包括16个不同的系统发育组（A-P）。这些UGT组具有不同的分子量，亚细胞定位和进化速率。种内共线性分析进一步表明，莲花UGT家族的扩展主要是由串联重复和全基因组重复驱动的。此外，对来自10种不同莲花组织的54个RNA-seq样品的表达分析表明，大多数UGT家族成员的表达表现出组织或发育阶段特异性，并且在生殖器官（组织）中表达的UGT比在营养器官中表达的UGT进化得快得多。更有趣的是，关键的UGT基因之间的差异调节温带和热带莲花（生态型）在幼苗期被发现，表明UGT与激素的合成和运输可能有助于莲花生态型之间的早期分化。总体而言，我们的系统表达和进化分析不仅表征了UGT家族在水生植物中的表达，功能多样性和进化动态，而且还提供了深入了解UGT家族在荷花生态型分化中的作用。
Family-1 UDP-glycosyltransferases, designated as UGTs, glycosylate key cellular metabolites into compounds with improved stability, solubility and transport to regulate plant growth and development in various metabolic pathways. However, little is known about the molecular and functional characteristics of UGT family in an aquatic plant and how it contributes to the Nelumbo nucifera ecotypic differentiation. Therefore, we performed a genome-wide investigation of 108 candidate UGT genes distributing on all eight lotus chromosomes, encompassing 16 distinct phylogenetic groups (A-P). These UGT groups feature different molecular weights, subcellular localizations and evolutionary rates. Intra-specific collinearity analysis further suggests that the expansion of the UGT family in lotus is mainly driven by tandem duplication and whole-genome duplication. Further, expression analysis of 54 RNA-seq samples from ten different lotus tissues suggests that expression of most UGT family members exhibits tissue or developmental-stage specificity, and UGTs expressed in reproductive organs (tissues) evolve much faster than those expressed in vegetative organs. More interestingly, key UGT genes being differentially regulated between temperate and tropical lotus (ecotypes) were uncovered during the seedling stage, indicating that UGTs associated with hormone synthesis and transport likely contribute to early differentiation between lotus ecotypes. Overall, our systematic expression and evolutionary analyses not only characterize the expression, functional diversity and evolutionary dynamics of the UGT family in an aquatic plant but also provide insight into the roles of the UGT family underlying the differentiation of the lotus ecotypes.