DNase II digestion of the nucleosome core: precise locations and relative exposures of sites.
DNase II digestion of the nucleosome core: precise locations and relative exposures of sites.
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核小体核心的 DNase II 消化：精确位置和位点的相对暴露。
DOI：
10.1093/nar/9.17.4251
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发表时间：
1981
影响因子：
14.9
通讯作者：
L. Lutter
中科院分区：
文献类型：
--
作者：
L. Lutter
核小体核心 DNA 中 DNase II 可接近位点的精确位置和相对暴露是使用先前用于 DNase I 酶的技术来确定的。发现两种酶的位点暴露模式之间存在许多相似之处，但一般来说 DNase II 似乎比 DNase I 更少区分相邻位点的可接近性。这两种酶攻击 DNA 中基本相同的位置，位点精确位置之间的平均差异小于两种酶的二分之一碱基。两种具有不同裂解机制的酶的消化模式如此相似，表明这些模式反映了核小体核心的结构，而不仅仅是所用特定酶的特性。
The precise locations and relative exposures of the DNase II-accessible sites in the nucleosome core DNA are determined using techniques previously employed for the enzyme DNase I. It is found that there are a number of similarities between the site exposure patterns for the two enzymes but that in general the DNase II seems to discriminate less among adjacent sites' accessibilities than does DNase I. The two enzymes attack essentially the same positions in the DNA, the average difference between the precise location of the site being less than one-half base for the two enzymes. Such close similarities in the digestion patterns of two enzymes with such different mechanisms of scission show that the patterns reflect the structure of the nucleosome core and not merely the properties of the particular enzyme used.