Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation
Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation
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DOI：
10.1104/pp.104.057935
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发表时间：
2005-06-01
期刊：
影响因子：
7.4
通讯作者：
Becker, JD
中科院分区：
文献类型：
--
作者：
Pina, C;Pinto, F;Becker, JD
在萌发时，花粉形成一个管状体，通过雌性组织到达并使胚珠受精。虽然对高等植物的生命周期至关重要，但大多数过程背后的遗传基础尚不清楚。我们之前使用流式细胞术对活的水合花粉颗粒进行分类，并对拟南芥（拟南芥）基因组的三分之一进行基因芯片阵列分析，以确定花粉转录组的第一个概述。现在，我们使用Affymetrix拟南芥ATH1阵列将该研究扩展到拟南芥基因组的约80%，并对花粉和营养组织转录组中的基因家族和基因本体表示进行了比较分析。与任何营养组织相比，花粉粒具有更小且整体独特的转录组（表达6587个基因），选择性表达（11%）和富集（26%）基因的比例更高。花粉和营养组织中的相关基因本体论类别表示揭示了花粉转录组向信号传导、囊泡运输和细胞骨架的功能倾斜，暗示了萌发和管状生长的承诺。细胞周期分析揭示了G2/ m相关因子的积累可能在合子的第一次有丝分裂中发挥作用。尽管转录相关转录物的代表性相对不足，但非经典MADS盒子基因作为一类在花粉中具有假定的独特作用的基因出现。成熟花粉粒中基因表达控制的单一性进一步突出了小RNA途径组分的明显缺失。
Upon germination, pollen forms a tube that elongates dramatically through female tissues to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We previously used a combination of flow cytometry sorting of viable hydrated pollen grains and GeneChip array analysis of one-third of the Arabidopsis ( Arabidopsis thaliana) genome to define a first overview of the pollen transcriptome. We now extend that study to approximately 80% of the genome of Arabidopsis by using Affymetrix Arabidopsis ATH1 arrays and perform comparative analysis of gene family and gene ontology representation in the transcriptome of pollen and vegetative tissues. Pollen grains have a smaller and overall unique transcriptome ( 6,587 genes expressed) with greater proportions of selectively expressed ( 11%) and enriched ( 26%) genes than any vegetative tissue. Relative gene ontology category representations in pollen and vegetative tissues reveal a functional skew of the pollen transcriptome toward signaling, vesicle transport, and the cytoskeleton, suggestive of a commitment to germination and tube growth. Cell cycle analysis reveals an accumulation of G2/ M-associated factors that may play a role in the first mitotic division of the zygote. Despite the relative underrepresentation of transcription- associated transcripts, nonclassical MADS box genes emerge as a class with putative unique roles in pollen. The singularity of gene expression control in mature pollen grains is further highlighted by the apparent absence of small RNA pathway components.