Connexin 43 transfection in basaloid squamous cell carcinoma cells.
Connexin 43 transfection in basaloid squamous cell carcinoma cells.
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DOI：
10.3892/or.16.2.285
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发表时间：
2006-08
期刊：
影响因子：
4.2
通讯作者：
K. Shima;T. Muramatsu;Y. Abiko;Y. Yamaoka;Hodaka Sasaki;M. Shimono
中科院分区：
文献类型：
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作者：
K. Shima;T. Muramatsu;Y. Abiko;Y. Yamaoka;Hodaka Sasaki;M. Shimono
为了探讨连接蛋白43(Cx43)在基底细胞样鳞癌(BSCC)中的表达与其快速增殖和侵袭潜能的关系，我们检测了Cx43过表达对BSCC来源细胞系(BSC-OF)的影响。将Cx43导入BSC-OF，获得15个稳定表达Cx43的克隆。在这些细胞中，虽然Cx43分布在细胞质中，但没有形成连接蛋白斑块。在几乎所有的克隆中，细胞增殖明显受到抑制。此外，我们研究了三个细胞克隆的细胞迁移和侵袭，发现Cx43基因对这三个克隆的细胞生长均没有显著影响。这些结果表明，Cx43在转导Cx43的BSC-Of细胞胞浆中起到了肿瘤抑制作用。然而，未发现Cx43与细胞的迁移和侵袭有明确的相关性。
To investigate the relationship between the expression of connexin in basaloid squamous cell carcinomas (BSCC) and their rapid proliferation and invasive potential, we examined the effect of overexpression of connexin 43 (Cx43) in a BSCC-derived cell line (BSC-OF). BSC-OF was transfected with Cx43 to obtain 15 clones with a stable expression of Cx43. In these cells, although Cx43 was distributed throughout the cytoplasm, it did not form connexon plaque. In almost all of the clones, cell proliferation was clearly suppressed. Furthermore, we investigated cell migration and invasion in three clones that showed a remarkable down-regulation in cell growth, and found that Cx43 transfection showed no significant effect on either. These results suggest that Cx43 plays a role as a tumor suppressor in the cytoplasm of Cx43-transfected BSC-OF cells. However, no definite correlation was found between Cx43 and cell migration and invasion.