Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein
Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein
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DOI：
10.1093/humrep/dei012
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发表时间：
2005-08-01
影响因子：
6.1
通讯作者：
Taketani, Y
中科院分区：
文献类型：
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作者：
Hirata, T;Osuga, Y;Taketani, Y
背景技术背景：为了改善子宫内膜异位症的动物模型，我们开发了一个同源小鼠模型，使用“绿色小鼠”，广泛表达绿色荧光蛋白。方法：将从补充雌二醇（E-2）的卵巢切除的“绿色小鼠”获得的子宫内膜片段切碎并注射到卵巢切除的野生型小鼠的腹膜腔中。受体野生小鼠在补充或不补充E-2的情况下饲养2周，然后安乐死。在荧光下对腹部发生的子宫内膜异位病变进行肉眼和显微镜检查，并测量病变重量。结果：荧光成像比常规肉眼检查能更清楚地检测到肿瘤病变。从组织学上看，荧光显微镜下可以清楚地区分来自“绿色小鼠”的子宫内膜异位病变与周围宿主组织。补充E-2的小鼠比对照受体小鼠出现更多病变。荧光强度与体重呈显著正相关（R=0.844，P < 0.0001），且E-2组明显高于溶剂组（P=0.0062）。结论：目前使用“绿色小鼠”的子宫内膜异位症模型将有助于快速鉴定和定量评价子宫内膜异位症病变。
BACKGROUND: Aiming at improving an animal model of endometriosis, we developed a homologous mouse model using 'green mice' that ubiquitously express green fluorescent protein. METHODS: Endometrial fragment obtained from estradiol (E-2)-supplemented ovariectomized 'green mice' was minced and injected into the peritoneal cavity of ovariectomized wild-type mice. The recipient wild mice were raised with or without E-2 supplementation for 2 weeks, and then were euthanized. Endometriotic lesions that developed in the abdomen were examined both macroscopically and microscopically under fluorescence, and weight of the lesions was measured. RESULTS: The endometriotic lesions were more clearly detected under fluorescence imaging than by conventional macroscopic examination. Histologically, endometriotic lesions deriving from 'green mice' were sharply distinguished from surrounding host tissues under fluorescence microscopy. More lesions developed in E-2-supplemented than control recipient mice. The measured fluorescence intensity of endometriotic lesions showed significant positive correlation with their weight (R=0.844, P < 0.0001), and was significantly higher in E-2-supplemented mice than in vehicle-supplemented mice (P=0.0062). CONCLUSION: The present endometriosis model using 'green mice' would be useful for expeditious identification and quantitative evaluation of endometriotic lesions.