Recognition of oxytocin and oxytocin-related peptides in aqueous media using a molecularly imprinted polymer synthesized by the epitope approach
Recognition of oxytocin and oxytocin-related peptides in aqueous media using a molecularly imprinted polymer synthesized by the epitope approach
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DOI：
10.1016/s0021-9673(00)00568-9
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发表时间：
2000-08-11
影响因子：
4.1
通讯作者：
Minoura, N
中科院分区：
文献类型：
--
作者：
Rachkov, A;Minoura, N
通过分子印迹的表位方法制备的人工聚合物受体被证明在水介质中识别肽激素催产素。所提出的方法是基于使用（作为模板）的化合物，其结构代表了一个较大分子的一个小的暴露片段（作为表位代表抗原）。高效液相色谱研究表明，离子相互作用和N-末端氨基的催产素和催产素相关肽的分子印迹聚合物在富水介质中的识别过程中的重要作用。然而，认为该工艺的特异性由疏水相互作用和氢键决定。此外，它表明，分子印迹聚合物的选择性可以衰减的水含量，离子强度和pH值的色谱移动的相：根据这些因素的模板，Tyr-Pro-Leu-Gly-NH，或，例如，催产素，一个较大的肽，它具有相同的三个氨基酸的C-末端部分的结构，可以优先保留的分子印迹聚合物。(C)2000 Elsevier Science B. V.保留所有权利。
An artificial polymeric receptor prepared by the epitope approach of molecular imprinting was shown to recognize the peptide hormone, oxytocin, in aqueous media. The proposed approach is based on using (as a template) a compound, whose structure represents a small exposed fragment of a larger molecule (as an epitope represents an antigen). A HPLC study has demonstrated the important role of ionic interactions and the N-terminal amino group of oxytocin and oxytocin-related peptides in the process of their recognition by the molecularly imprinted polymer in the aqueous-rich media. However, the specificity of the process is considered to be defined by hydrophobic interactions and hydrogen bonding. Moreover, it was shown that the selectivity of the molecularly imprinted polymer can be attenuated by water content, ionic strength and pH of the chromatographic mobile phase: depending on these factors the template, Tyr-Pro-Leu-Gly-NH,, or, for example, oxytocin, a larger peptide, which possesses the same three amino-acid C-terminal parts of the structure, can be preferentially retained by the molecularly imprinted polymer. (C) 2000 Elsevier Science B.V. All rights reserved.