Accumulation of phosphatidic acid mass and increased de novo synthesis of glycerolipids in platelet-activating-factor-activated human neutrophils.
Accumulation of phosphatidic acid mass and increased de novo synthesis of glycerolipids in platelet-activating-factor-activated human neutrophils.
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血小板激活因子激活的人中性粒细胞中磷脂酸质量的积累和甘油脂的从头合成增加。
DOI：
10.1042/bj2800625
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Venkatesh
中科院分区：
文献类型：
--
作者：
J. Tou;J. R. Jeter;C. Dola;S. Venkatesh
用100 nM血小板活化因子（PAF）孵育人中性粒细胞，但不加细胞松弛素B，导致磷脂酸（PtdOH）质量快速（5 s）积累（1.6倍）。增加PtdOH质量达到最大值（2.8倍），在1分钟，并保持升高（1.7倍），在10分钟。没有甲胺稳定的溶血PtdOH是检测到的总脂质提取物从控制或PAF激活的细胞，这表明二酰基PtdOH是占主导地位的物种。在PAF激活的细胞中，在5或15秒时未检测到1，2-二酰甘油（DG）质量的变化。增加DG质量（1.7倍）之间检测到30秒和2分钟，但随后下降到基础水平的10分钟。PAF增强[3 H]甘油掺入PtdOH和DG的2-和3-倍，分别在1-10分钟孵育。PAF也增加了放射性，但不是质量的磷脂酰肌醇和胆碱甘油磷脂的8倍和4倍，分别在10分钟。此外，PAF激活的细胞表现出增加（2倍）甘油掺入三酰甘油。这些结果表明，PAF增强二酰基-PtdOH质量的快速积累，并且增加的从头合成可能有助于PtdOH质量积累。
Incubation of human neutrophils with 100 nM-platelet-activating factor (PAF) but without cytochalasin B resulted in a rapid (5 s) accumulation (1.6-fold) of phosphatidic acid (PtdOH) mass. The increased PtdOH mass reached a maximum (2.8-fold) at 1 min and remained elevated (1.7-fold) at 10 min. No methylamine-stable lyso-PtdOH was detectable in the total lipid extract from control or from PAF-activated cells, suggesting that diacyl-PtdOH was the predominant species. In PAF-activated cells, changes in 1,2-diacylglycerol (DG) mass were not detectable at 5 or 15 s. Increased DG mass (1.7-fold) was detected between 30 s and 2 min, but then it declined to basal levels by 10 min. PAF enhanced [3H]glycerol incorporation into PtdOH and DG by 2- and 3-fold respectively during 1-10 min incubations. PAF also increased the radioactivity but not the mass of phosphatidylinositol and of choline glycerophospholipid by 8-fold and 4-fold respectively at 10 min. In addition, PAF-activated cells showed increased (2-fold) glycerol incorporation into triacylglycerol. These results demonstrate that PAF enhances rapid accumulation of diacyl-PtdOH mass, and that increased de novo synthesis may contribute to PtdOH mass accumulation.