Capillary electrophoresis and electrochemical detection with a conventional detector cell
Capillary electrophoresis and electrochemical detection with a conventional detector cell
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DOI：
10.1016/s0021-9673(97)00964-3
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发表时间：
1998-03-06
影响因子：
4.1
通讯作者：
Kok, WT
中科院分区：
文献类型：
--
作者：
Durgbanshi, A;Kok, WT
对一种市售电化学检测池的性能进行了评价，以用于毛细管电泳（CE）。结果表明，在适当安装毛细管的情况下，由电池引起的区域展宽受到电极表面分析物损耗的限制。尽管细胞的几何体积相对较大，但其对区域宽度的贡献仅为1-2秒。当耦合到CE装置时，可以获得高达100,000的板号。对于直径为1 -mm的玻碳电极，探测器噪声为3-5 pA，取决于所施加的分离电压。在此噪声级下，可以得到10(-8)mol l（-1）的检测限。该细胞已用于测定儿茶酚胺、氯酚类和邻苯二醛类氨基酸衍生物。(C) 1998爱思唯尔科学有限公司
The performance of a commercially available electrochemical detector cell has been evaluated for use in capillary electrophoresis (CE). It was shown that with proper installation of the capillary, the zone broadening caused by the cell is limited by the depletion of the analytes on the electrode surface. Despite the relatively large geometric volume of the cell, its contribution to zone widths was therefore only in the order of 1-2 s. When coupled to a CE set-up, plate numbers up to 100 000 could be obtained. With a I-mm diameter glassy carbon electrode the detector noise was 3-5 pA, depending on the applied separation voltage. With this noise level detection limits of 10(-8) mol l(-1) could be obtained. The cell has been used for the determination of catecholamines, chlorophenols and o-phthalaldehyde derivatives of amino acids. (C) 1998 Elsevier Science B.V.