Experiments on a sub-unit vaccine encapsulated in microparticles and its efficacy against Brucella melitensis in mice.
Experiments on a sub-unit vaccine encapsulated in microparticles and its efficacy against Brucella melitensis in mice.
复制标题
微粒封装亚单位疫苗及其对小鼠布鲁氏菌功效的实验。
DOI：
10.1016/j.vaccine.2006.01.038
复制
发表时间：
2006
期刊：
影响因子：
5.5
通讯作者：
J. Irache
中科院分区：
文献类型：
--
作者：
M. Estevan;C. Gamazo;M. Grilló;G. del Barrio;J. Blasco;J. Irache
本研究的目的是评价用于促进从布鲁氏菌（HS）中提取的高疏水抗原复合物的包封的辅料对所得到的微粒的物理化学性质的影响。采用全循环一体机系统（TROMS），采用溶剂萃取/蒸发法制备了含HS的聚己内酯（PEC）微粒。采用不同的辅料β-环糊精（β-CD）、Pluronic®F68、Tween 20或Tween 80，以促进包封和保持包封抗原复合物的生物活性。HS有效地装载在所有不同的pec -微颗粒配方中，尽管β-环糊精和Pluronic®F68的联合使用允许在得到的微颗粒的核心中增加抗原提取物的量而不损失其抗原特性。最后，在BALB/c小鼠中，对该F68-CD-MP制剂对毒力强的梅利氏布鲁氏菌H38菌株的实验攻击进行了评估。这种无害的亚细胞疫苗产生了与Rev 1减毒活疫苗相似的保护作用；这些都是有希望的结果，值得在目标动物中进一步研究。
The aim of this study was to evaluate the effect of the excipients used to facilitate the encapsulation of high hydrophobic antigenic complex extracted from Brucella ovis (HS) on the physico-chemical properties of the resulting microparticles. Poly(ɛ-caprolactone) (PEC) microparticles containing HS were prepared by the solvent extraction/evaporation method using total recirculation one-machine system (TROMS). Different excipients, β-cyclodextrin (β-CD), Pluronic®F68, Tween 20 or Tween 80, were used in order to facilitate the encapsulation and conserve the bioactivity of the encapsulated antigenic complex. HS was efficiently loaded in all the different PEC-microparticle formulations, although the combined use of β-cyclodextrin and Pluronic®F68 permitted an increase in the amount of antigenic extract in the core of the resulting microparticles without loss of its antigenic properties. Finally, the protective ability of this F68–CD–MP formulation was evaluated against an experimental challenge with the virulent Brucella melitensis H38 strain in BALB/c mice. This innocuous subcellular vaccine induced a similar protection to that of the live attenuated Rev 1 vaccine; these are promising results that would merit further investigation in target animals.