Regulatory CD4+and CD8+T cells are negatively correlated with CD4+/CD8+T cell ratios in patients acutely infected with SARS-CoV-2
Regulatory CD4+and CD8+T cells are negatively correlated with CD4+/CD8+T cell ratios in patients acutely infected with SARS-CoV-2
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DOI：
10.1002/jlb.5cova0720-421rr
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发表时间：
2020-09-15
影响因子：
5.5
通讯作者：
Zhu, Zhaoqin
中科院分区：
文献类型：
--
作者：
Gao, Menglu;Liu, Yili;Zhu, Zhaoqin
调节性 T 细胞可以防止宿主炎症反应引起的严重冠状病毒感染。但其在 COVID-19 发病机制中的作用仍不清楚。在这项研究中，使用多色流式细胞术分析了 COVID-19 患者和出院个体外周血中淋巴细胞总数和多个亚群的频率。使用基于微球的免疫测定试剂盒测量 IL-10 的血浆浓度。与健康对照相比，急性感染的 COVID-19 患者和出院个体的总淋巴细胞和 T 细胞频率均显着下降。总淋巴细胞的频率与CD3(-)CD56(+)NK细胞的频率呈负相关。调节性CD8(+)CD25(+)T细胞的频率与CD4(+)/CD8(+)T细胞比率呈正相关，而调节性CD4(+)CD25(+)CD127(-)T细胞的频率与CD4(+)/CD8(+)T细胞比率呈负相关。 45岁以上患者的CD4(+)/CD8(+)T细胞比率显着增加。相应地，这些患者中调节性CD8(+)CD25(+)T细胞的频率也显着增加。总的来说，结果表明调节性 CD4+ 和 CD8+T 细胞可能在 COVID-19 的发病机制中发挥不同的作用。此外，数据表明 NK 细胞可能导致 COVID-19 相关的淋巴细胞减少症。
Regulatory T cell can protect against severe forms of coronaviral infections attributable to host inflammatory responses. But its role in the pathogenesis of COVID-19 is still unclear. In this study, frequencies of total and multiple subsets of lymphocytes in peripheral blood of COVID-19 patients and discharged individuals were analyzed using a multicolor flow cytometry assay. Plasma concentration of IL-10 was measured using a microsphere-based immunoassay kit. Comparing to healthy controls, the frequencies of total lymphocytes and T cells decreased significantly in both acutely infected COVID-19 patients and discharged individuals. The frequencies of total lymphocytes correlated negatively with the frequencies of CD3(-)CD56(+)NK cells. The frequencies of regulatory CD8(+)CD25(+)T cells correlated with CD4(+)/CD8(+)T cell ratios positively, while the frequencies of regulatory CD4(+)CD25(+)CD127(-)T cells correlated negatively with CD4(+)/CD8(+)T cell ratios. Ratios of CD4(+)/CD8(+)T cells increased significantly in patients beyond age of 45 years. And accordingly, the frequencies of regulatory CD8(+)CD25(+)T cells were also found significantly increased in these patients. Collectively, the results suggest that regulatory CD4(+)and CD8(+)T cells may play distinct roles in the pathogenesis of COVID-19. Moreover, the data indicate that NK cells might contribute to the COVID-19 associated lymphopenia.