Application of Hyperbaric Oxygen Reduce Oxidative Damage of Plasmatic Carbonyl Proteins and 8-OHdG by Activating Glutathion Peroxidase
Application of Hyperbaric Oxygen Reduce Oxidative Damage of Plasmatic Carbonyl Proteins and 8-OHdG by Activating Glutathion Peroxidase
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DOI：
10.7754/clin.lab.2014.140929
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发表时间：
2015-01-01
影响因子：
0.7
通讯作者：
Smolle-Juettner, Freyja-Maria
中科院分区：
文献类型：
--
作者：
Matzi, Veronika;Greilberger, Joachim F.;Smolle-Juettner, Freyja-Maria
背景：据推测，高压氧的应用可能会在高压氧后诱导自由基的产生。更高的氧合和氧运输会增加线粒体能量周转，而线粒体内部自由基形成会减少。方法：在健康志愿者 (n = 21) 的氧化应激之前、期间和之后测量几种氧化应激标志物，包括血浆羰基蛋白 (CP)、丙二醛 (MDA)、氧化低密度脂蛋白 (oxLDL)、8-羟基脱氧鸟苷 (8-OHdG) 和红细胞谷胱甘肽过氧化物酶 (GPx) 活性HBO 结果：与 HBO 之前的 CP 水平相比，HBO 期间 CP 的中位血浆浓度显着降低（从 77.1 降至 61.7 pmol/mg；p < 0.001），并且在 HBO 后再次升高（至 78.1 pmol/mg；p = 0.035）。与“HBO 之前”(9.4 ng/mL) 相比，8-OHdG 在 HBO 期间显着下降 (8.1 ng/mL；p < 0.001)，并且在 HBO 后保持恒定 (8.1 ng/mL)。 MDA从0.92μM（HBO前）显着增加至1.26μM（HBO期间，p <0.01），并再次下降至1.00μM（HBO后，p = 0.023）。 HBO 期间红细胞 GPx 活性也显着增加（26.5 +/- 14.7；p = 0.005），但 HBO 后则没有显着增加（25.6 +/- 17.2 IU/mg）。仅在 HBO 期间观察到 GPx 和 MDA 之间呈负相关（r = -0.518；p = 0.016）。结论：我们假设较高的耗氧量一方面会减少线粒体内自由基的产生，另一方面会通过激活 GPx 等解毒酶来减少 RONS。
Background: It is postulated that application of hyperbaric oxygenation may induce the production of radicals after HBO. Higher oxygenation and transport of oxygen increase the mitochondrial energy turnover, whereas inner mitochondrial radical formation decreases.Methods: Several markers of oxidative stress in healthy volunteers (n = 21), including plasma carbonyl proteins (CP), malondialdehyde (MDA), oxidized LDL (oxLDL), 8-hydroxy-deoxyguanosine (8-OHdG), and erythrocyte glutathione peroxidase (GPx) activity are measured before, during, and after HBO.Results: Median plasma concentrations of CP decreased significantly during HBO compared to CP levels before HBO (from 77.1 to 61.7 pmol/mg; p < 0.001) and increased again after HBO (to 78.1 pmol/mg; p = 0.035). 8-OHdG decreased significantly during HBO (8.1 ng/mL; p < 0.001) and remained constant after HBO (8.1 ng/mL) compared to "before HBO" (9.4 ng/mL). MDA increased significantly from 0.92 mu M (before HBO) to 1.26 mu M (during HBO, p < 0.01) and decreased again to 1.00 mu M (after HBO, p = 0.023). Erythrocyte GPx activity also increased significantly during HBO (26.5 +/- 14.7; p = 0.005), but not after HBO (25.6 +/- 17.2 IU/mg). A negative correlation was observed between GPx and MDA only during HBO (r = -0.518; p = 0.016).Conclusions: We assume that higher oxygen consumption decreases, on the one hand, the inner mitochondrial generation of free radicals and, on the other, RONS by activation of detoxifying enzymes like GPx.