Sustained Release of VEGF by Coaxial Electrospun Dextran/PLGA Fibrous Membranes in Vascular Tissue Engineering
Sustained Release of VEGF by Coaxial Electrospun Dextran/PLGA Fibrous Membranes in Vascular Tissue Engineering
复制标题
血管组织工程中同轴电纺右旋糖酐/PLGA纤维膜持续释放VEGF
DOI：
10.1163/092050610x528534
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Jia, Xiaoling;Zhao, Chenguang;Fan, Yubo
分别以葡聚糖（DEX）为核组分、聚（丙交酯-共-乙交酯）（PLGA）为壳聚合物，采用同轴静电纺丝法制备了载VEGF的核/壳纤维膜。通过扫描电镜、透射电镜和共聚焦显微镜观察DEX/PLGA电纺纤维的核/壳结构和蛋白质分布。拉伸实验结果表明，DEX/PLGA膜的拉伸强度低于PLGA膜，而杨氏模量高于PLGA膜。释放曲线表明，血管内皮生长因子（VEGF）释放持续超过28天。细胞活性和铺展性研究表明，DEX（VEGF）/PLGA膜对细胞增殖和细胞膜相互作用有积极的促进作用，进一步证明了处理后的VEGF仍具有生物活性。此外，在病理刺激下检测到与炎症过程和血栓形成相关的细胞间粘附分子-1的上调和von Willebrand因子的释放，对DEX（VEGF）/PLGA膜表现出正常的免疫应答。这些数据表明，负载VEGF的纤维在血管组织工程中是可行的。(C)Koninklijke Brill NV，莱顿，2011年
VEGF-loaded core/shell fibrous membranes were prepared by coaxial electrospinning with dextran (DEX) as the core component and poly(lactide-co-glycolide) (PLGA) as the shell polymer, respectively. The electrospun DEX/PLGA fibers were observed by scanning electron microscopy, transmission electron microscopy and confocal microscopy to identify the core/shell fiber structure and the protein distribution. The results of tensile tests showed that the DEX/PLGA membranes possessed lower tensile strength and higher Young's modulus than PLGA one. The release profiles demonstrated that vascular endothelial growth factor (VEGF) release sustained for more than 28 days. Studies on cell viability and spreading demonstrated that the DEX(VEGF)/PLGA membranes positively promoted cell proliferation and cell-membrane interaction, which further testified that the processed VEGF remained bioactivities. Furthermore, the detections for the up-regulation of intercellular adhesion molecular-1 and the release of von Willebrand factor under pathological stimuli, which are related to inflammation process and thrombus formation, exhibited a normal immune response for the DEX(VEGF)/PLGA membrane. These data suggested that the VEGF-loaded fibers could be feasible in vascular tissue engineering. (C) Koninklijke Brill NV, Leiden, 2011