Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides
Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides
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DOI：
10.1039/c8lc01291a
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发表时间：
2019-03-21
期刊：
影响因子：
6.1
通讯作者：
Gleeson, Helen F.
中科院分区：
文献类型：
--
作者：
Bao, Peng;Paterson, Daniel A.;Gleeson, Helen F.
我们描述了一种新的生物传感器的基础上磷脂涂层的双相液晶（LC）液滴和演示检测Smp 43，一个模型抗菌肽（AMP）从北非蝎子蝎maurus palmatus毒液。使用具有流动聚焦配置的PDMS微流体装置产生直径为16.7 +/-0.2 μ m的单分散脂质涂覆的LC液滴，并且其是AMP的靶标。将液滴捕获在定制的微流体阱结构中，并在六个不同的室中以梯度浓度用Smp 43同时处理。检测到Smp 43对脂质单层的破坏（
We describe a novel biosensor based on phospholipid-coated nematic liquid crystal (LC) droplets and demonstrate the detection of Smp43, a model antimicrobial peptide (AMP) from the venom of North African scorpion Scorpio maurus palmatus. Mono-disperse lipid-coated LC droplets of diameter 16.7 +/- 0.2 m were generated using PDMS microfluidic devices with a flow-focusing configuration and were the target for AMPs. The droplets were trapped in a bespoke microfluidic trap structure and were simultaneously treated with Smp43 at gradient concentrations in six different chambers. The disruption of the lipid monolayer by the Smp43 was detected (