Label-Free Ultrathin Pt Film Biosensor Using Phonon Vibrations Excited by Ultrafast Light Pulses
Label-Free Ultrathin Pt Film Biosensor Using Phonon Vibrations Excited by Ultrafast Light Pulses
复制标题
利用超快光脉冲激发声子振动的无标记超薄铂膜生物传感器
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
M. Hirao
中科院分区：
文献类型：
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作者：
H. Ogi;Kozo Matsumoto;Y. Fujita;T. Kawamoto;N. Nakamura;M. Hirao
介绍了一种用于高灵敏度监测溶液中生物分子间结合反应的压电薄膜振荡式生物传感器。用超快光脉冲测量了18 nm Pt膜的声子沿着厚度方向的振动，其基本共振频率为120 GHz。在人免疫球蛋白G（hIgG）和抗hIgG抗体之间的结合反应期间监测振动振幅，其随着反应进行呈指数下降。可检测到6 pM hIgG溶液。该生物传感器还显示出通过反应速率的浓度依赖性来确定蛋白质之间的亲和力的能力。
An ultrathin film oscillator biosensor is presented for highly sensitive monitoring of binding reactions among biomolecules in solutions. The phonon vibration along the thickness direction of an 18-nm Pt film is measured by ultrafast light pulses, whose fundamental resonance frequency is 120 GHz. The vibrational amplitude is monitored during binding reaction between human immunoglobulin G (hIgG) and anti-hIgG antibody, which exponentially decreases as the reaction progresses. A 6 pM hIgG solution can be detected. This biosensor also shows the ability to determine the affinity between proteins through the concentration dependence of the reaction rate.
影响因子：
7.4
作者：
Nedelkov, Dobrin
通讯作者：
Nedelkov, Dobrin