A standardized protocol for the rapid preparation of bacterial DNA for pulsed-field gel electrophoresis
A standardized protocol for the rapid preparation of bacterial DNA for pulsed-field gel electrophoresis
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DOI：
10.1016/s0732-8893(98)00007-8
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发表时间：
1998-05-01
影响因子：
2.9
通讯作者：
Chui, L
中科院分区：
文献类型：
--
作者：
Chang, N;Chui, L
建立了一种快速提取革兰氏阳性菌和革兰氏阴性菌脉冲场凝胶电泳DNA的方法。该方法可将细胞裂解反应、限制性内切酶消化和电泳时间分别缩短至1、1.5和18 h。从最初的细菌培养板到最终的限制性片段分析的整个过程可以在24 h内完成。这种快速方法成功地实现了金黄色葡萄球菌，粪肠球菌，淋病奈瑟菌，鼠伤寒沙门氏菌，粘质沙雷氏菌，嗜麦芽窄食单胞菌。(C)1998年爱思唯尔科学公司
A rapid method for the preparation of bacterial DNA for pulsed-field gel electrophoresis was developed for Gram-positive and Gram-negative bacteria. This method mns accomplished by reducing the time for the cell lysis reaction, restriction endonuclease digestion, and electrophoresis to 1, 1.5, and 18 h, respectively. The whole procedure from the initial bacterial culture plate to the final analysis of restriction fragments can be completed within 24 h. This rapid method was successfully achieved for Staphylococcus aureus, Enterococcus faecalis, Neisseria gonorrhoeae, Salmonella typhimurium, Serratia marcescens, and Stenotrophomonas maltophilia. (C) 1998 Elsevier Science Inc.