Urinary tract infection caused by a small colony variant form of capnophilic Escherichia coli leading to misidentification and non-reactions in antimicrobial susceptibility tests
Urinary tract infection caused by a small colony variant form of capnophilic Escherichia coli leading to misidentification and non-reactions in antimicrobial susceptibility tests
复制标题
由嗜二氧化碳大肠杆菌的小菌落变体形式引起的尿路感染导致抗菌药物敏感性试验中的错误识别和无反应
DOI：
--
复制
发表时间：
2018
影响因子：
5.5
通讯作者：
D. Yong
中科院分区：
文献类型：
--
作者：
Yu Jin Park;Nguyen Le Phuong;N. Pinto;M. Kwon;Roshan D’Souza;Jung;H. Sung;D. Yong
小菌落和嗜二氧化碳性变异病例已分别报告，但尚未有报告称它们同时存在于一个分离株中。我们报告一例大肠杆菌与共表达的小菌落和嗜二氧化碳表型造成错误鉴定的自动生化试剂盒和抗菌药物敏感性测试卡的非反应。一名86岁女性因大肠杆菌菌株导致尿路感染，该菌株同时具有SCV和嗜二氧化碳表型。该菌株在不存在CO2的情况下不生长，因此不可能从自动化系统中进行适当鉴定。16 s rRNA测序和基质辅助激光解吸/电离飞行时间质谱能够鉴定细菌。由于这些菌株不能在CLSI规定的培养参数或自动化系统上生长，因此有必要使用替代方法进行适当鉴定。
Small colony and capnophilic variant cases have been separately reported, but there has been no reports of their simultaneous presence in one isolate. We report a case of Escherichia coli with coexpressed small colony and capnophilic phenotypes causing misidentification in automated biochemical kits and non-reactions in antimicrobial susceptibility test cards. An 86-year-old woman developed urinary tract infection from a strain of Escherichia coli with SCV and capnophilic phenotypes in co-existence. This strain did not grow without the presence of CO2, and therefore proper identification from automated system was not possible. 16 s rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was able to identify the bacteria. As these strains do not grow on culture parameters defined by CLSI or on automated systems, proper identification using alternative methods are necessary.