CHARACTERIZATION OF TRICHOMONAS-VAGINALIS HEMOLYSIS
CHARACTERIZATION OF TRICHOMONAS-VAGINALIS HEMOLYSIS
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DOI：
10.1017/s0031182000063204
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发表时间：
1990-10-01
期刊：
影响因子：
2.4
通讯作者：
ALDERETE, JF
中科院分区：
文献类型：
--
作者：
DAILEY, DC;CHANG, TH;ALDERETE, JF
研究了活的阴道毛滴虫生物体的溶血活性。在 2 小时的潜伏期内，寄生虫与红细胞的比例为 1:5，观察到人红细胞的最佳溶血情况。滴虫过夜生长后或当通过3μm过滤器将寄生虫与红细胞分离时，在浓缩培养物上清液中未检测到溶血活性，这表明溶血的接触依赖性机制。溶血活性依赖于温度并且最大溶血发生在37°C。用甲硝唑治疗滴虫可将溶血水平降低 50% 以上。滴虫感染期间，最大溶血发生在阴道的 pH 范围内。 N-α-甲苯磺酰-L-赖氨酰-氯甲基酮和碘乙酰胺，毛滴虫半胱氨酸蛋白酶抑制剂，降低活寄生虫的溶血活性。
The haemolytic activity of live Trichomonas vaginalis organisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 .mu.m filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37.degree.C. Treatment of trichomonads with metronidazole reduced levels of haemolysis by > 50%. Maximal haemolysis occurred at the pH range of the vagina during trichomoniasis. N-.alpha.-tosyl-L-lysyl-chloromethyl ketone and iodoacetamide, inhibitors of trichomonad cysteine proteinases, reduced the haemolytic activity of live parasites.