Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body.
Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body.
复制标题
DOI：
10.1083/jcb.200308132
复制
发表时间：
2004-01-19
影响因子：
7.8
通讯作者：
Rosenbaum, Joel L
中科院分区：
文献类型：
--
作者：
Qin, Hongmin;Diener, Dennis R;Geimer, Stefan;Cole, Douglas G;Rosenbaum, Joel L
鞭毛内转运（IFT）是多亚基蛋白颗粒沿着轴丝微管的双向运动，是真核生物鞭毛和纤毛组装和维持所必需的。IFT的一个假定作用是将鞭毛前体运输到鞭毛尖端进行组装。在这里，我们研究径向辐条，轴丝亚基组成的22个多肽，作为潜在的货物IFT。径向辐条被发现是部分组装在细胞体，然后被运送到鞭毛尖端的顺行IFT。完全组装的径向辐条，脱离轴丝微管在鞭毛破裂或营业额，从鞭毛逆行IFT。IFT颗粒，电机，径向辐条，和其他轴丝蛋白之间的相互作用进行了验证，这些蛋白质从衣原体鞭毛的可溶性部分的coimmunoprecipitation。这些研究表明，IFT在鞭毛组装和维持中的主要作用之一是运输轴丝蛋白进出鞭毛。
Intraflagellar transport (IFT) is the bidirectional movement of multisubunit protein particles along axonemal microtubules and is required for assembly and maintenance of eukaryotic flagella and cilia. One posited role of IFT is to transport flagellar precursors to the flagellar tip for assembly. Here, we examine radial spokes, axonemal subunits consisting of 22 polypeptides, as potential cargo for IFT. Radial spokes were found to be partially assembled in the cell body, before being transported to the flagellar tip by anterograde IFT. Fully assembled radial spokes, detached from axonemal microtubules during flagellar breakdown or turnover, are removed from flagella by retrograde IFT. Interactions between IFT particles, motors, radial spokes, and other axonemal proteins were verified by coimmunoprecipitation of these proteins from the soluble fraction of Chlamydomonas flagella. These studies indicate that one of the main roles of IFT in flagellar assembly and maintenance is to transport axonemal proteins in and out of the flagellum.