The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p
The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p
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DOI：
10.1128/mcb.20.16.5939-5946.2000
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发表时间：
2000-08-01
影响因子：
5.3
通讯作者：
Lapeyre, B
中科院分区：
文献类型：
--
作者：
Bonnerot, C;Boeck, R;Lapeyre, B
我们在此报告了 pab1 Delta 旁路抑制子的特征，该抑制子使不稳定的 MFA2 mRNA 四倍稳定。该抑制子的野生型基因的克隆表明，它与 PAT1 (YCR077c) 相同，据报道该基因的产物与 Top2p 相互作用，PAT1 不是必需基因，但其缺失会导致热敏表型。进一步分析表明，PAT1 与 mrt1-3 等位基因，mrt1-3 是先前报道的一种突变，会影响脱帽并绕过抑制 pab1 Delta，如下所示 dcp1、spb8 和 mrt3 的情况也是如此，免疫共沉淀实验表明 Pat1p 与 Spb8p 相关，在蔗糖梯度上，两种蛋白与含有多聚核糖体的级分共沉淀，然而，在没有 Pat1p 的情况下，Spb8p 不再与多聚核糖体共分级，而 Spb8p 的去除导致多聚核糖体水平急剧下降。 帕特1p。我们的结果表明，参与 mRNA 降解的一些因素可能与仍在翻译的 mRNA 相关，等待特定信号将 mRNA 投入降解途径。
We report here the characterization of a bypass suppressor of pab1 Delta which leads to a fourfold stabilization of the unstable MFA2 mRNA. Cloning of the wild-type gene for that suppressor reveals that it is identical to PAT1 (YCR077c), a gene whose product was reported to interact with Top2p, PAT1 is not an essential gene, but its deletion leads to a thermosensitive phenotype, Further analysis has shown that PAT1 is allelic with mrt1-3, a mutation previously reported to affect decapping and to bypass suppress pab1 Delta, as is also the case for dcp1, spb8, and mrt3, Coimmunoprecipitation experiments show that Pat1p is associated with Spb8p, On sucrose gradients, the two proteins cosediment with fractions containing the polysomes, In the absence of Pat1p, however, Spb8p no longer cofractionates with the polysomes, while the removal of Spb8p leads to a sharp decrease in the level of Pat1p. Our results suggest that some of the factors involved in mRNA degradation could be associated with the mRNA that is still being translated, awaiting a specific signal to commit the mRNA to the degradation pathway.