Role of abscisic acid in perianth senescence of daffodil (Narcissus pseudonarcissus 'Dutch Master')
Role of abscisic acid in perianth senescence of daffodil (Narcissus pseudonarcissus 'Dutch Master')
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DOI：
10.1111/j.0031-9317.2004.0311.x
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发表时间：
2004-06-01
影响因子：
6.4
通讯作者：
Reid, MS
中科院分区：
文献类型：
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作者：
Hunter, DA;Ferrante, A;Reid, MS
水仙（Narcissus pseudonarcissus L.）‘荷兰大师’)花在子房基部分离，在10-100 muM脱落酸（ABA）过早衰老。ABA处理的症状包括被片浸水和冠层早期塌陷。浸泡在水中的花的花被没有被水浸透。相反，这些花的花被片干了。随着盆栽花的衰老，花被片中ABA含量逐渐增加。花被片ABA含量的上升与视觉衰老迹象的出现一致。当花被剪下并放入水中，这是一种加速其衰老的处理，ABA的增加发生得更早。外源外源ABA促进了被片衰老相关转录物的提前积累。当花被1-甲基环丙烯（一种乙烯作用抑制剂）预处理时，ABA介导的诱导并没有被阻止，这表明ABA诱导转录本独立于乙烯。在内源ABA增加之前，转录本在开放的对照花中积累。利用ABA生物合成抑制剂[钨酸盐、氟酮（用作Sonar(R)）和1,1-二甲基-4-（苯基磺酰基）氨基脲（DPSS）]延长花寿命的尝试均未成功。然而，在花瓶溶液中加入100 muM赤霉素酸（GA(3)）可以降低50 muM ABA的衰老诱导作用，这可能是体内控制衰老的一种机制。
Daffodil (Narcissus pseudonarcissus L. 'Dutch Master') flowers detached at the base of their ovaries and held with their cut ends in 10-100 muM abscisic acid (ABA) senesced prematurely. Symptoms of the ABA treatment included water-soaking of the tepals and early collapse of the corona. No water-soaking was seen in tepals of flowers held in water. Instead, the tepals of these flowers dried. The ABA content increased in tepals of the potted flowers as they senesced. The rise in tepal ABA content coincided with the appearance of visual signs of senescence. When the flowers were cut and placed in water, a treatment that accelerated their senescence, the increase in ABA occurred earlier. Exogenously applied ABA enhanced the premature accumulation of senescence-associated transcripts in the tepals. Their ABA-mediated induction was not prevented when the flowers were pre-treated with 1-methylcyclopropene, an inhibitor of ethylene action, indicating that ABA induced the transcripts independently of ethylene. The transcripts accumulated in opened control flowers before the rise in endogenous ABA. Attempts to extend floral longevity by using putative inhibitors of ABA biosynthesis [tungstate, fluridone (applied as Sonar(R)) and 1,1-dimethyl-4-(phenylsulphonyl)semicarbazide (DPSS)] were unsuccessful. However, inclusion of 100 muM gibberellic acid (GA(3)) in the vase solution reduced the senescence-inducing effects of 50 muM ABA suggesting a possible mechanism for in-vivo control of senescence.