Osteoblast precursor cells are found in CD34(+) cells from human bone marrow
Osteoblast precursor cells are found in CD34(+) cells from human bone marrow
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DOI：
10.1002/stem.150368
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发表时间：
1997-01-01
期刊：
影响因子：
5.2
通讯作者：
Choi, ESH
中科院分区：
文献类型：
--
作者：
Chen, JL;Hunt, P;Choi, ESH
众所周知，成骨前体细胞存在于人骨髓（BM）抽吸物的低密度单核（LDMN）部分中。本研究的目的是研究造血祖细胞标志物CD34是否存在于成骨祖细胞上。LDMN、CD34（+）和CD34（-）细胞在促进矿物质分泌成骨细胞生长和分化的条件下以有限稀释的方式培养。在LDMN细胞中，成骨祖细胞的平均频率为1/36,000个细胞。对于CD34（-）细胞，成骨细胞祖细胞频率保持在平均1/33,000，与LDMN细胞相似。在CD34（+）选择的细胞中，成骨细胞祖细胞的频率增加到平均1/ 5000。在分选的CD34(+)/CD38（+）细胞中，这种成骨细胞祖细胞的频率保持不变。CD34（+）细胞生成的成骨细胞形态正常，地塞米松诱导分化后骨特异性碱性磷酸酶和骨连接素表达增加。在超微结构上，这些CD34（+）细胞衍生的成骨细胞表现出成骨细胞的特异性特征。功能上，这些CD34（+）细胞衍生的成骨细胞在DEX处理下分化，在甲状旁腺激素刺激下增加环腺苷单磷酸水平，增加碱性磷酸酶活性水平，增加矿物质分泌。这些结果表明，成骨细胞祖细胞在BM的CD34（+）细胞群中富集，这些祖细胞可以在培养中分化为功能性成骨细胞。
It is known that osteoblast precursor cells are found in the low-density mononuclear (LDMN) fraction of human bone marrow (BM) aspirates. The purpose of this study was to investigate whether CD34, a hematopoietic progenitor cell marker, is present on osteoblast progenitor cells. LDMN, CD34(+), and CD34(-) cells were cultured under conditions that promote growth and differentiation of mineral-secreting osteoblasts in a limiting dilution manner. With LDMN cells, osteoblast progenitor cells were found at an average frequency of 1/36,000 cells. With CD34(-) cells, osteoblast progenitor frequency remained at an average of 1/33,000, similar to LDMN cells. With CD34(+) selected cells, osteoblast progenitor frequency increased to an average of 1/5,000. This osteoblast progenitor frequency is maintained in sorted CD34(+)/CD38(+) cells. The osteoblasts generated from CD34(+) cells were morphologically normal, and expression of skeletal-specific alkaline phosphatase and osteonectin increased upon differentiation induced by dexamethasone (DEX) treatment. Ultrastructurally, these CD34(+) cell-derived osteoblasts displayed osteoblast-specific features. Functionally, these CD34(+) cell-derived osteoblasts differentiated with DEX treatment, increased the level of cyclic adenosine monophosphate in response to parathyroid hormone stimulation, increased the level of alkaline phosphatase activity, and increased mineral secretion. These results demonstrate that osteoblast progenitor cells are enriched in the CD34(+) cell population from BM and that these progenitor cells can differentiate into functional osteoblasts in culture.