Usefulness of the hermaphroditic marine fish, Rivulus marmoratus, in carcinogenicity testing.
Usefulness of the hermaphroditic marine fish, Rivulus marmoratus, in carcinogenicity testing.
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雌雄同体海水鱼 Rivulus marmoratus 在致癌性测试中的用途。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
Chasar Mp
中科院分区：
文献类型：
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作者：
Koenig Cc;Chasar Mp
在水中暴露于15、30和45 ppm的二乙基亚硝胺（DENA）可诱导自受精雌雄同体的marmoratus鱼发生肝细胞癌和其他病理变化。暴露12周的幼鱼和暴露5周的成鱼在1年后的检查中显示，肝细胞癌的发生率分别高达76.5%和31.3%。将处于不同发育阶段的胚胎鱼暴露在10、32、100、320和1,000 ppm的DENA中一周，一年后检查时没有肿瘤。其他病理改变为胆管瘤、腺纤维化和肉芽肿，发生率分别高达38.9%、88.9和61.1%。本研究开发了简单的饲养方法，并讨论了沙鼠繁殖、胚胎发育和后续生长的相关生物学资料。评估了使用马氏鼠作为致癌性试验动物的利弊。
: Hepatocellular carcinoma and other pathological changes were induced in the self-fertilizing hermaphroditic fish Rivulus marmoratus by aqueous exposure to 15, 30, and 45 ppm diethylnitrosamine (DENA). Fish exposed as larvae for 12 weeks and adults exposed for 5 weeks showed rates of hepatocellular carcinoma as high as 76.5 and 31.3%, respectively, when examined 1 year later. Fish exposed as embryos in various stages of development for 1 week to 10, 32, 100, 320, and 1,000 ppm DENA were without neoplasms when examined 1 year later. Other pathological changes of rivulus livers were cholangioma, adenofibrosis, and granuloma with incidences as high as 38.9, 88.9, and 61.1%, respectively. Simple rearing methods were developed and relevant biological data pertaining to reproduction, embryonic development, and subsequent growth of R. marmoratus are discussed. Advantages and disadvantages of the use of R. marmoratus as a carcinogenicity test animal are assessed.