SENSITIVE COLLOIDAL METAL (GOLD OR SILVER) STAINING OF PROTEIN BLOTS ON NITROCELLULOSE MEMBRANES
SENSITIVE COLLOIDAL METAL (GOLD OR SILVER) STAINING OF PROTEIN BLOTS ON NITROCELLULOSE MEMBRANES
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DOI：
10.1016/0003-2697(85)90368-9
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
DEMEY, J
中科院分区：
文献类型：
--
作者：
MOEREMANS, M;DANEELS, G;DEMEY, J
描述了一种在硝化纤维素膜上进行蛋白质印迹的灵敏染色方法，并与常用的染料染色方法进行了比较。它使用胶体金属溶胶（金或银）稳定与Tween 20和调整到pH值3。它是基于胶体金属颗粒与蛋白质的选择性高亲和力结合，并产生红紫色（金色）或深灰色（银色）。这种新染色方法的灵敏度与聚丙烯酰胺凝胶的银染色在同一范围内，并且与覆盖法的灵敏度相匹配。因此，它将是一种有用的工具，用于将覆盖法检测到的蛋白质条带或斑点的位置与重复蛋白质印迹中的完整电泳图相关联。
A sensitive staining method for protein blots on nitrocellulose membranes is described and compared with commonly used dye staining methods. It uses colloidal metal sols (gold or silver) stabilized with Tween 20 and adjusted to pH 3. It is based on the selective high-affinity binding of colloidal metal particles to the proteins and produces a red-purplish color (gold) or dark grey (sliver). The sensitivity of this new staining method is in the same range as silver staining of polyacrylamide gels and matches the sensitivity of overlay assays. It will therefore be a useful tool for correlating the position of bands or spots of proteins detected with overlay assays with the complete electropherogram in a duplicate protein blot.