The wheat TaIQD3D-6 gene encodes a microtubule-associated protein and regulates cell morphogenesis in Arabidopsis
The wheat TaIQD3D-6 gene encodes a microtubule-associated protein and regulates cell morphogenesis in Arabidopsis
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小麦 TaIQD3D-6 基因编码微管相关蛋白并调节拟南芥细胞形态发生
DOI：
10.1016/j.plantsci.2022.111420
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发表时间：
2022-08-22
期刊：
影响因子：
5.2
通讯作者：
Liu, Xiayan
中科院分区：
文献类型：
--
作者：
Liu, Haofeng;Cao, Yongxin;Liu, Xiayan
大量的微管相关蛋白（MAPs）调节微管（MT）的动力学，以确保植物发育程序的正确阐述。在植物特异性MAP中有IQ 67结构域（IQD）家族蛋白。尽管在阐明IQD蛋白的功能方面取得了很大进展，但大多数IQD蛋白，特别是作物物种中的IQD，仍有待于对其功能进行探索。在这项研究中，我们确定了78个推定的IQD家族基因的六倍体小麦（小麦）的基因组。小麦和拟南芥IQD的系统发育分析支持了先前的观点，即IQD家族的扩展与植物的陆生化相一致。进一步表征一种TaIQD，TaIQD 3D-6，揭示TaIQD 3D-6在体外直接结合MT和游离微管蛋白，并且在体内与间期细胞中的皮质MT相关。在拟南芥中过表达TaIQD 3D-6导致了一系列表型，这些表型表明MT稳态受到干扰，包括螺旋生长、对MT不稳定药物的超敏反应、细胞形态发生缺陷以及cMT阵列的组织改变。最后，我们确定了TaIQD 3D-6-GFP定位于胞质分裂过程中扩展的细胞板，并且TaIQD 3D-6的过表达干扰了拟南芥气孔谱系中的不对称细胞分裂。总之，我们的研究结果确定TaIQD 3D-6是一种MAP，调节植物细胞和器官形态发生，并为作物IQD蛋白的功能提供了新的见解。
A plethora of microtubule-associated proteins (MAPs) modulate the dynamics of microtubules (MTs) to ensure the proper elaboration of developmental programs in plants. Among the plant-specific MAPs are the IQ67 domain (IQD) family proteins. Despite the great progress in elucidating IQD protein functions, the majority of IQD proteins, especially IQDs in crop species, remain to be functionally explored. In this study, we identified 78 putative IQD family genes in the genome of hexaploid wheat (Triticum aestivum). Phylogenetic analysis of wheat and Arabidopsis IQDs supports the previous notion that the expansion of the IQD family coincides with plant terrestrialization. Further characterization of one TaIQD, TaIQD3D-6, revealed that TaIQD3D-6 directly binds to MTs and free tubulins in vitro and is associated with cortical MTs in interphase cells in vivo. Overexpressing TaIQD3D-6 in Arabidopsis leads to a spectrum of phenotypes that are indicative of perturbed MT homeostasis, including spiral growth, hypersensitivity to MT-destabilizing drugs, defects in cell morphogenesis, and altered organization of cMT arrays. Finally, we determined that TaIQD3D-6-GFP localizes to the expanding cell plate during cytokinesis and the overexpression of TaIQD3D-6 interferes with asymmetric cell division in the stomatal lineage in Arabidopsis. In summary, our findings establish that TaIQD3D-6 is a MAP that regulates plant cell and organ morphogenesis and provide new insights into the functions of crop IQD proteins.