Odorant receptors instruct functional circuitry in the mouse olfactory bulb
Odorant receptors instruct functional circuitry in the mouse olfactory bulb
复制标题
DOI：
10.1038/nature01001
复制
发表时间：
2002-09-19
期刊：
影响因子：
64.8
通讯作者：
Katz, LC
中科院分区：
文献类型：
--
作者：
Belluscio, L;Lodovichi, C;Katz, LC
哺乳动物的嗅觉系统使用鼻上皮中感觉神经元表达的许多不同受体来检测和区分数千种气味（1）。从这些神经元到主要嗅球的轴突投射在每个嗅球的两个分开的区域中形成了可重复的肾小球模式，产生了两个镜像对称的气味受体投射图（2）。为了研究气味受体是否组织嗅球中的神经回路，我们检查了一种遗传修饰小鼠系rI 7--> M71，其中功能特征受体rI 7（3，4）已被替换到M71受体基因座中（5）。在这里，我们表明，尽管他们的异位位置所产生的肾小球是响应于已知的配体的rI 7受体，吸引突触后神经支配的二尖瓣/簇状细胞树突，并赋予这些细胞的反应是rI 7受体的特征。接受来自rI 7--> M71肾小球输入的外部簇状细胞形成精确的球内投射，其在解剖学上连接内侧和外侧rI 7--> M71肾小球，从而为协调同功能肾小球提供基底。我们的结论是，上皮神经元的气味受体的身份不仅决定肾小球的收敛和功能，但也功能电路的嗅球。
The mammalian olfactory system detects and discriminates thousands of odorants using many different receptors expressed by sensory neurons in the nasal epithelium(1). Axonal projections from these neurons to the main olfactory bulbs form reproducible patterns of glomeruli in two widely separated regions of each bulb, creating two mirror-symmetric maps of odorant receptor projections(2). To investigate whether odorant receptors organize neural circuitry in the olfactory bulb, we have examined a genetically modified mouse line, rI7 --> M71, in which a functionally characterized receptor, rI7(3,4), has been substituted into the M71 receptor locus(5). Here we show that despite their ectopic location the resulting glomeruli are responsive to known ligands of the rI7 receptor, attract postsynaptic innervation by mitral/tufted cell dendrites, and endow these cells with responses that are characteristic of the rI7 receptor. External tufted cells receiving input from rI7 --> M71 glomeruli form precise intrabulbar projections that link medial and lateral rI7 --> M71 glomeruli anatomically, thus providing a substrate for coordinating isofunctional glomeruli. We conclude that odorant receptor identity in epithelial neurons determines not only glomerular convergence and function, but also functional circuitry in the olfactory bulb.