Minimally-invasive transepidermal potentiometry with microneedle salt bridge
Minimally-invasive transepidermal potentiometry with microneedle salt bridge
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DOI：
10.1007/s10544-016-0080-0
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发表时间：
2016-08-01
影响因子：
2.8
通讯作者：
Nishizawa, Matsuhiko
中科院分区：
文献类型：
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作者：
Abe, Yuina;Nagamine, Kuniaki;Nishizawa, Matsuhiko
将一种商用的无痛微针填充生理盐水琼脂，将这种针状盐桥插入皮肤(一片猪皮和一只活鼠的侧皮)，与其真皮下区域进行电接触。使用该内电极和皮肤表面的常规琼脂电极测量了皮肤表面和真皮下区域之间的跨皮电位(TEP)。为了稳定测量，用隔离物控制内电极的穿透深度和用臭氧等离子体进行亲水前处理是必要的。通过剥离胶带和丙酮脱脂对皮肤表面的损伤，降低了TEP，表明该电极可用于TEP的微创测量和皮肤屏障功能的评价。此外，我们还表明，将两个电极集成到一个紧凑的探针中的装置对于评估局部屏障功能及其在皮肤上的映射是有用的。该设备有望成为未来医学和化妆品领域的一种个人诊断工具。
A commercial painless microneedle was filled with physiological saline agar, and this needle-based salt bridge was inserted into the skin (a piece of porcine skin and a flank skin of a live mouse) to make an electrical contact with its subepidermal region. The transepidermal potential (TEP), the potential difference between the skin surface and the subepidermal region, was measured using this inner electrode and a conventional agar electrode on the surface of the skin. Control of penetration depth of the inner electrode with a spacer and hydrophilic pretreatment with ozone plasma were found to be necessary for stable measurement. The TEP was reduced upon damages on the skin surface by tape stripping and acetone defatting, which indicated the fabricated needle electrode is useful for the minimally-invasive measurement of TEP and evaluation of skin barrier functions. Furthermore, we showed that the device integrating two electrodes into a single compact probe was useful to evaluate the local barrier functions and their mapping on a skin. This device could be a personal diagnostic tool in the fields of medicine and cosmetics in future.