The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes
The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes
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DOI：
10.1016/j.ygcen.2011.10.011
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Larhammar, Dan
中科院分区：
文献类型：
--
作者：
Daza, Daniel Ocampo;Lewicka, Michalina;Larhammar, Dan
脊椎动物的催产素和加压素受体形成一个G蛋白偶联受体(GPCRs)家族，介导多种功能，包括社会行为和血压、水分平衡和生殖的调节。在哺乳动物中，已鉴定出四个家族成员，其中三个对加压素(VP)有反应，分别为V1a、V1B和V2，其中一个被催产素(OT)激活。被称为OT受体。在鸡肉中也发现了四种受体，但它们的名字不同。直到最近，在几种硬骨鱼中只有V1型受体被描述。我们已经在几个节口类基因组中确定了家族成员，并进行了系统发育分析，以跨物种对OT/VP-受体进行分类，并确定直系关系。我们的系统发育树确定了OT/VP受体家族中五种不同的祖传Gnathostome受体亚型：VIA、V1B、V2A、V2B和OT受体。不同的V2a和V2b受体的存在还没有被认识到。我们在所有被研究的硬骨鱼基因组以及现有的青蛙和蜥蜴基因组中都发现了这两个亚型，并得出结论：V2A型与哺乳动物的V2受体同源，而V2B型与鸟类的V2受体同源。一些硬骨鱼已经获得了额外的和更新的基因重复，最多可有8个受体家族成员。因此，这一分析揭示了Gathostome中OT/VP受体的前所未有的复杂性，开启了关于水平衡、繁殖和行为调节等功能的有趣研究途径，特别是在爬行动物、两栖动物、硬骨鱼和软骨鱼中。(C)2011 Elsevier Inc.保留所有权利。
The vertebrate oxytocin and vasopressin receptors form a family of G-protein-coupled receptors (GPCRs) that mediate a large variety of functions, including social behavior and the regulation of blood pressure, water balance and reproduction. In mammals four family members have been identified, three of which respond to vasopressin (VP) named V1A, V1B and V2, and one of which is activated by oxytocin (OT). called the OT receptor. Four receptors have been identified in chicken as well, but these have received different names. Until recently only V1-type receptors have been described in several species of teleost fishes. We have identified family members in several gnathostome genomes and performed phylogenetic analyses to classify OT/VP-receptors across species and determine orthology relationships. Our phylogenetic tree identifies five distinct ancestral gnathostome receptor subtypes in the OT/VP receptor family: VIA, V1B, V2A, V2B and OT receptors. The existence of distinct V2A and V2B receptors has not been previously recognized. We have found these two subtypes in all examined teleost genomes as well as in available frog and lizard genomes and conclude that the V2A-type is orthologous to mammalian V2 receptors whereas the V2B-type is orthologous to avian V2 receptors. Some teleost fishes have acquired additional and more recent gene duplicates with up to eight receptor family members. Thus, this analysis reveals an unprecedented complexity in the gnathostome repertoire of OT/VP receptors, opening interesting research avenues regarding functions such as regulation of water balance, reproduction and behavior, particularly in reptiles, amphibians, teleost fishes and cartilaginous fishes. (C) 2011 Elsevier Inc. All rights reserved.