SILVER STAIN FOR DETECTING 10-FEMTOGRAM QUANTITIES OF PROTEIN AFTER POLYACRYLAMIDE-GEL ELECTROPHORESIS
SILVER STAIN FOR DETECTING 10-FEMTOGRAM QUANTITIES OF PROTEIN AFTER POLYACRYLAMIDE-GEL ELECTROPHORESIS
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DOI：
10.1016/0003-2697(83)90703-0
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
EBATA, N
中科院分区：
文献类型：
--
作者：
OHSAWA, K;EBATA, N
建立了一种快速、高灵敏度的银染色法和彩色染色法。手术过程简单，条带清晰。银染色可检测100pg量的蛋白质。为了快速、灵敏和清晰地染色凝胶中的蛋白质基质，用高、低渗溶液使凝胶反复收缩和膨胀，以去除SDS-蛋白质复合物中的十二烷基硫酸钠（SDS），并使染色溶液流入凝胶中。银染色法反复暴露于高、低渗溶液和窄孔产生10 fg级的蛋白质。
A rapid and highly sensitive silver stain and color stain were developed for visualizing proteins. The procedure is simple and the bands were clear. This silver stain detects 100 pg quantities of proteins. In order to stain quickly, sensitively and sharply a protein matrix in a gel, repeated shrinking and swelling of the gel were caused by hyper- and hypotonic solutions to remove the sodium dodecyl sulfate (SDS) from SDS-protein complex and to generate influx of staining solution into the gel. The silver staining method with repeated exposure to hyper- and hypotonic solution and a narrow well produced 10 fg order of proteins were found.