Selenomethylene Locked Nucleic Acid Enables Reversible Hybridization in Response to Redox Changes
Selenomethylene Locked Nucleic Acid Enables Reversible Hybridization in Response to Redox Changes
复制标题
硒亚甲基锁定核酸可实现可逆杂交以响应氧化还原变化
DOI：
10.1002/anie.201300555
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kunihiko Morihiro
中科院分区：
文献类型：
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作者：
Dwyer SA;Chow WS;Yamori W. Evans JR;Kaines S;Badger MR & von Caemmerer S;Kunihiko Morihiro
锁定硒：一种新的具有2′，4′-硒亚甲基桥的构象限制性核酸（SeLNA）可以可逆地转化为其氧化形式（SeOLNA），并且修饰的寡核苷酸的杂交显示出依赖于氧化态。SeLNA修饰的分子信标型探针（参见方案; F=荧光团，Q=猝灭剂）可以用作氧化还原环境变化的传感器。
Locking up selenium: A new conformationally restricted nucleic acid with a 2′, 4′‐selenomethylene bridge (SeLNA) can be reversibly converted into its oxidized form (SeOLNA), and the hybridization of a modified oligonucleotide was shown to be dependent on the oxidation state. A SeLNA‐modified molecular‐beacon‐type probe (see scheme; F= fluorophore, Q= quencher) can be used as a sensor for changes in the redox environment.