Mass spectrometric quantification of cysteine-containing leukotrienes in rat bile using 13C-labeled internal standards.
Mass spectrometric quantification of cysteine-containing leukotrienes in rat bile using 13C-labeled internal standards.
复制标题
使用 13C 标记的内标对大鼠胆汁中含有半胱氨酸的白三烯进行质谱定量。
DOI：
10.1002/bms.1200211008
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Gaskell,SJ
中科院分区：
文献类型：
--
作者：
Raftery,MJ;Justesen,U;Jaeschke,H;Gaskell,SJ
采用高效液相色谱分离、5-羟基二十烷酸加氢、气相色谱/质谱选择离子监测分析五氟苯甲酯、三甲基硅烷醚衍生物的方法，测定了大鼠胆汁中的白三烯C4和N-乙酰白三烯E4。麻醉动物注射内毒素后，胆汁中两种白三烯的浓度均显著升高，其中N-乙酰白三烯E4含量较高。用快原子轰击/串联质谱仪结合前驱体离子扫描，证实内毒素攻击后大鼠胆汁中存在白三烯C4。用串联质谱法对白三烯C4进行定量测定与气相色谱/质谱法测定结果一致，但后一种方法的检出限大大降低。
Leukotriene C4andN‐acetyl leukotriene E4were determined in rat bile using a procedure based on high‐performance liquid chromatographic fractionation, hydrogenation to 5‐hydroxyeicosanoic acid, and gas chomatographic/mass spectrometric selected ion monitoring analysis of the pentafluorobenzyl ester, trimethylsilyl ether derivatives.13C4‐Labeled analogs of the leukotrienes were synthesized and used as internal standards. The concentrations of both leukotrienes in rat bile were markedly elevated after administration of endotoxin to anesthetized animals;N‐acetyl leukotrieneE4was the more abundant product. The presence of leukotriene C4in rat bile after endotoxin challenge was confirmed by fast atom bombardment/tandem mass spectrometry with precursor ion scanning. Quantitative determination of leukotriene C4using the tandem mass spectrometric procedure was consistent wth the gas chromatographic/mass spectrometric data but the latter procedure gave a substantially lower detection limit.