ENANTIOMER LABELING, A METHOD FOR THE QUANTITATIVE-ANALYSIS OF AMINO-ACIDS
ENANTIOMER LABELING, A METHOD FOR THE QUANTITATIVE-ANALYSIS OF AMINO-ACIDS
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DOI：
10.1016/s0021-9673(00)91157-9
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发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
BAYER, E
中科院分区：
文献类型：
--
作者：
FRANK, H;NICHOLSON, GJ;BAYER, E
对映体标记是一种通过气相色谱法定量分析光学活性天然化合物的方法，涉及使用非天然对映体作为内标物。手性固定相Chirasil-Val在240° C下具有良好的热稳定性，可用于分离和定量测定氨基酸和其他多种化合物的对映体。通过加入非天然对映异构体，可以补偿样品中的不完全回收、不完全衍生、衍生物的水解和热分解以及响应因子的变化。通过对映体标记的氨基酸分析的准确性等于或上级于迄今已知的方法。该程序提供了肽的氨基酸组成和每种氨基酸的光学纯度的完整分析。
Enantiomer labelling, a method for the quantitative analysis of opticaly active natural compounds by gas chromatography, involves theuse of the unnatural enantiomer as an internal standard. WithChirasil-Val, a chiral stationary phase that is thermally stable up to up to 240°, the enantiomers of amino acid and a variety of other compounds can be separated and quantitated. Incomplete recovery from the sample, incomplete derivatization, hydrolysis and thermal decomposition of the derivative and shifting response factors can be compensated for by adding the unnatural enantiomer. The accuracy of amino acid analysis by enantiomer labelling is equal or superior to that of hitherto known methods. The procedure affords a complete analysis of peptides with respect to both amino acid composition and the optical purity of each amino acid.