UV Inactivation of SARS-CoV-2 across the UVC Spectrum: KrCl* Excimer, Mercury-Vapor, and Light-Emitting-Diode (LED) Sources.
UV Inactivation of SARS-CoV-2 across the UVC Spectrum: KrCl* Excimer, Mercury-Vapor, and Light-Emitting-Diode (LED) Sources.
复制标题
DOI：
10.1128/aem.01532-21
复制
发表时间：
2021-10-28
影响因子：
4.4
通讯作者：
Linden KG
中科院分区：
文献类型：
--
作者：
Ma B;Gundy PM;Gerba CP;Sobsey MD;Linden KG
抗击严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的有效消毒技术可以帮助减少正在进行的新冠肺炎全球大流行期间和未来的病毒传播。发射紫外线照射(200至280 nm)的紫外线装置已被证明对病毒消毒有效，但由于检测的安全要求，可获得的关于SARS-CoV-2的信息有限，仅限于生物安全3级实验室。在这项研究中，测定了薄膜缓冲水溶液(pH 7.4)中的SARS-CoV-2在2 2 2~2 82 nm的紫外线照射波长范围内的灭活情况，这些紫外线来自氯化氪准分子、一个低压汞蒸汽灯和两个紫外线发光二极管。我们的结果表明，所有被测试的紫外线装置都能有效地灭活SARS-CoV-2，其中KRCL*准分子的消毒性能最好(即灭活率最高)。SARS-CoV-2的跨波长灭活速率常数与我们先前研究中的小鼠肝炎病毒(MHV)的灭活速率常数相似，表明MHV在紫外线消毒试验中可以作为BSL要求(BSL2)较低的SARS-CoV-2的可靠替代品。这项研究为紫外线对SARS-CoV-2的作用提供了基本信息，并为紫外线装置实现可靠的消毒性能提供了指导。重要性紫外线是一种有效的工具，有助于在商业、公共、交通和医疗保健环境中阻止呼吸道病毒的传播并保护公众健康。为了有效利用紫外线，需要确定不同紫外线波长在杀灭病原体，特别是SARS-CoV-2方面的效率，以支持正在进行的新冠肺炎全球大流行和未来由冠状病毒引起的呼吸道病毒大流行的控制工作。我们发现，使用广泛的紫外线波长可以有效地灭活SARS-CoV-2，其中222 nm的消毒效果最好。有趣的是，222纳米的辐射已经被发现对人类来说是安全的，直到超过了有效灭活病毒的阈值。因此，在公共场所使用KRCL*准分子的紫外线可以有效地减少病毒气溶胶或地面传播。
Effective disinfection technology to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can help reduce viral transmission during the ongoing COVID-19 global pandemic and in the future. UV devices emitting UVC irradiation (200 to 280 nm) have proven to be effective for virus disinfection, but limited information is available for SARS-CoV-2 due to the safety requirements of testing, which is limited to biosafety level 3 (BSL3) laboratories. In this study, inactivation of SARS-CoV-2 in thin-film buffered aqueous solution (pH 7.4) was determined across UVC irradiation wavelengths of 222 to 282 nm from krypton chloride (KrCl*) excimers, a low-pressure mercury-vapor lamp, and two UVC light-emitting diodes. Our results show that all tested UVC devices can effectively inactivate SARS-CoV-2, among which the KrCl* excimer had the best disinfection performance (i.e., highest inactivation rate). The inactivation rate constants of SARS-CoV-2 across wavelengths are similar to those for murine hepatitis virus (MHV) from our previous investigation, suggesting that MHV can serve as a reliable surrogate of SARS-CoV-2 with a lower BSL requirement (BSL2) during UV disinfection tests. This study provides fundamental information on UVC’s action on SARS-CoV-2 and guidance for achieving reliable disinfection performance with UVC devices. IMPORTANCE UV light is an effective tool to help stem the spread of respiratory viruses and protect public health in commercial, public, transportation, and health care settings. For effective use of UV, there is a need to determine the efficiency of different UV wavelengths in killing pathogens, specifically SARS-CoV-2, to support efforts to control the ongoing COVID-19 global pandemic and future coronavirus-caused respiratory virus pandemics. We found that SARS-CoV-2 can be inactivated effectively using a broad range of UVC wavelengths, and 222 nm provided the best disinfection performance. Interestingly, 222-nm irradiation has been found to be safe for human exposure up to thresholds that are beyond those effective for inactivating viruses. Therefore, applying UV light from KrCl* excimers in public spaces can effectively help reduce viral aerosol or surface-based transmissions.