The adjuvanticity of ophiopogon polysaccharide liposome against an inactivated porcine parvovirus vaccine in mice
The adjuvanticity of ophiopogon polysaccharide liposome against an inactivated porcine parvovirus vaccine in mice
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麦冬多糖脂质体对猪细小病毒灭活疫苗的小鼠佐剂作用
DOI：
10.1016/j.ijbiomac.2015.10.084
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发表时间：
2016-01-01
影响因子：
8.2
通讯作者：
Song, Xiaoping
中科院分区：
文献类型：
--
作者：
Fan, Yunpeng;Ma, Xia;Song, Xiaoping
本研究对麦冬多糖脂质体的佐剂活性进行了研究。体外检测OPL对小鼠脾淋巴细胞增殖的影响。结果表明，麦冬多糖单独或与PHA、LPS单独或协同作用均能显著促进淋巴细胞增殖，且在大部分浓度下均优于麦冬多糖(OP)。比较了OPL、OP和矿物油在BALB/c小鼠体内接种灭活PPV后的佐剂活性。结果表明，OPL能显著促进淋巴细胞增殖，提高CD4(+)、CD8(+)T细胞比例，提高HI抗体效价和特异性Ig G反应，促进细胞因子的产生，且OPL的疗效明显优于OP。此外，与油佐剂相比，OPL显著提高了细胞免疫应答。提示OPL具有较好的佐剂性，且中剂量效果最好。因此，OPL可作为PPV灭活疫苗的有效免疫佐剂。(C)由爱思唯尔出版的2015年。
In this study, the adjuvant activity of ophiopogon polysaccharide liposome (OPL) was investigated. The effects of OPL on the splenic lymphocyte proliferation of mice were measured in vitro. The results showed that OPL could significantly promote lymphocyte proliferation singly or synergistically with PHA and LPS and that the effect was better than ophiopogon polysaccharide (OP) at most of concentrations. The adjuvant activities of OPL, OP and mineral oil were compared in BALB/c mice inoculated with inactivated PPV in vivo. The results showed that OPL could significantly enhance lymphocyte proliferation, increase the proportion of CD4(+) and CD8(+) T cells, improve the HI antibody titre and specific IgG response, and promote the production of cytokines, and the efficacy of OPL was significantly better than that of OP. In addition, OPL significantly improved the cellular immune response compared with oil adjuvant. These results suggested that OPL possess superior adjuvanticity and that a medium dose had the best efficacy. Therefore, OPL can be used as an effective immune adjuvant for an inactivated PPV vaccine. (C) 2015 Published by Elsevier B.V.