A secretory protein restricted to type I cells in neonatal rat submandibular glands.
A secretory protein restricted to type I cells in neonatal rat submandibular glands.
复制标题
一种仅限于新生大鼠颌下腺 I 型细胞的分泌蛋白。
DOI：
10.1016/0012-1606(88)90393-4
复制
发表时间：
1988
影响因子：
2.7
通讯作者：
Johnson,AO
中科院分区：
文献类型：
--
作者：
Ball,WD;Hand,AR;Moreira,JE;Johnson,AO
大鼠的围产期颌下腺含有一种 89 kDa 的分泌蛋白（蛋白 C），在胆碱能刺激下释放。针对蛋白 C 产生的多克隆抗体表明，该蛋白位于 I 型细胞中，在典型的 III 型细胞中未发现。然而，III 型细胞（IIIP 型）的形态变异体含有与蛋白 C 抗体发生交叉反应的物质。在新生儿舌下腺、腮腺和小舌下腺以及成人下颌下腺和舌下腺的粘液细胞中也发现了交叉反应成分。电泳分离蛋白质的免疫印迹仅在新生儿颌下腺中显示出 89 kDa 处的独特蛋白质 C 带；新生儿舌下腺和小舌下腺在包括蛋白 C 在内的一系列活动范围内表现出一些弥漫性反应性。我们认为，粘液细胞和 IIIP 型细胞的交叉反应成分不是蛋白 C，而是与粘液分化相关的不同蛋白，并且新生儿颌下腺的 IIIP 型细胞正从 III 型向成熟粘液细胞转变。
The perinatal submandibular gland of the rat contains an 89-kDa secretory protein (Protein C) that is released upon cholinergic stimulation. Polyclonal antibodies raised against Protein C show that this protein is localized in the Type I cells and is not found in typical Type III cells. However, morphological variants of Type III cells (Type IIIP) contain material that is cross-reactive with antibodies to Protein C. Cross-reactive components also are found in mucous cells of the neonatal sublingual glands, parotid and minor sublingual glands, and adult submandibular and sublingual glands. Immunoblots of electrophoretically separated proteins show a distinct Protein C band at 89 kDa only in neonatal submandibular glands; neonatal sublingual and minor sublingual glands show some diffuse reactivity over a range of mobilities encompassing that of Protein C. We propose that the cross-reactive components of mucous cells and Type IIIP cells are not Protein C, but different proteins associated with mucous differentiation, and that the Type IIIP cells of the neonatal submandibular gland are in transition from Type III to mature mucous cells.