Near-Infrared Fluorescent Thiol-Organosilica Nanoparticles That Are Functionalized with IR-820 and Their Applications for Long-Term Imaging of in Situ Labeled Cells and Depth-Dependent Tumor in Vivo Imaging
Near-Infrared Fluorescent Thiol-Organosilica Nanoparticles That Are Functionalized with IR-820 and Their Applications for Long-Term Imaging of in Situ Labeled Cells and Depth-Dependent Tumor in Vivo Imaging
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DOI：
10.1021/acs.chemmater.0c01414
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发表时间：
2020-09-08
影响因子：
8.6
通讯作者：
Abe, Masahiro
中科院分区：
文献类型：
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作者：
Nakamura, Michihiro;Hayashi, Koichiro;Abe, Masahiro
通过一锅法制备了内部用IR-820官能化的硫醇-有机二氧化硅纳米颗粒（硫醇-OS/IR 820），用于近红外（NIR）荧光体内成像。Thiol-OS/IR 820具有宽发射光谱，具有多个不同于IR-820的新荧光峰，并且具有上转换荧光，具有生物相容性，在体内外均未显示出明显的毒性。我们对皮下异种移植细胞进行了原位标记细胞的体内追踪。体内成像显示原位标记的细胞向异种移植细胞的部位迁移和积聚。然后，3周后观察到移植细胞减少。接下来，我们使用静脉内施用硫醇-OS/IR 820对具有皮下异种移植肿瘤的小鼠进行体内肿瘤成像。使用三种波长的光发射，对具有皮下异种移植肿瘤的小鼠进行深度依赖性NIR荧光成像。由于增强的渗透性和保留（EPR）效应，观察到颗粒在肿瘤组织中的积累具有深度依赖性。使用thiol-OS/IR 820的近红外荧光体内成像可用于长期观察，并显示出对体内新生物现象可视化的实质性承诺。
Thiol-organosilica nanoparticles that are internally functionalized with IR-820 (thiol-OS/IR820) were prepared via a one-pot process for near-infrared (NIR) fluorescence in vivo imaging. Thiol-OS/IR820 demonstrated broad-band emissive NIR fluorescence with multiple new fluorescent peaks that differed from those of the IR-820 molecule and upconversion fluorescence originated from thiol-OS. Thiol-OS/IR820 was biocompatible and did not show significant toxicity in vitro and in vivo. We conducted in vivo tracking of in situ labeled cells against subcutaneous xenograft cells. The in vivo imaging showed a migration and an accumulation of the in situ labeled cells to the site of xenograft cells. Then, a reduction of the grafted cells was observed after 3 weeks. Next, we conducted in vivo tumor imaging of a mouse with a subcutaneous xenograft tumor using intravenous administration of thiol-OS/IR820. Using three wavelengths of light emission, depth-dependent NIR fluorescence imaging of a mouse with a subcutaneous xenograft tumor was conducted. The accumulation of particles in the tumor tissue due to the enhanced permeability and retention (EPR) effect was observed depth-dependently. NIR fluorescence in vivo imaging using thiol-OS/IR820 is useful for long-term observation and shows substantial promise for the visualization of novel biological phenomena in vivo.