In vivo quantification of quantum dot systemic transport in C57BL/6 hairless mice following skin application post-ultraviolet radiation.
In vivo quantification of quantum dot systemic transport in C57BL/6 hairless mice following skin application post-ultraviolet radiation.
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DOI：
10.1186/s12989-017-0191-7
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发表时间：
2017-04-14
影响因子：
10
通讯作者：
DeLouise LA
中科院分区：
文献类型：
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作者：
Jatana S;Palmer BC;Phelan SJ;Gelein R;DeLouise LA
先前的工作已经证明了纳米颗粒的大小、表面电荷和皮肤屏障依赖性渗透到小鼠皮肤的活性层中。这项工作的目的是表征组织分布和机制的纳米粒子的运输超过皮肤，有和没有紫外线辐射（UVR）诱导的皮肤屏障破坏。使用无毛C57 BL/6小鼠模型，使用原子吸收光谱（AAS）、流式细胞术和共聚焦显微镜检查UVR剂量（180和360 mJ/cm 2 UVB）对在UVR后不同时间点局部应用的量子点（QD）纳米颗粒的皮肤渗透和全身分布的影响。结果表明，量子点可以穿透小鼠皮肤，无论紫外线照射，证明了在所有QD治疗小鼠的局部淋巴结中的镉增加。所有处理组的平均回收率%为69.68%，约66.84%的给药剂量从皮肤（表皮和皮内）中回收。在各处理组中，从淋巴结中回收的平均剂量为0.024%。当在UV照射后4天应用QD时，在皮肤屏障缺陷和LC迁移至局部淋巴结的峰值，淋巴结中QD的细胞存在增加;然而，局部淋巴结的AAS分析显示，由于UVR处理，镉水平没有差异。我们的数据表明，朗格汉斯细胞（LC）可以吞噬皮肤中的量子点，但运输到淋巴结可能发生的细胞（树突状细胞和巨噬细胞）和非细胞机制。有趣的是，这些特定的纳米颗粒被保留在皮肤中类似，无论紫外线屏障破坏，但观察到的皮肤免疫细胞与纳米颗粒的相互作用表明免疫调节的潜力，我们目前正在研究的小鼠模型的皮肤过敏。本文的在线版本（doi：10.1186/s12989-017-0191-7）包含补充材料，可供授权用户使用。
Previous work has demonstrated size, surface charge and skin barrier dependent penetration of nanoparticles into the viable layers of mouse skin. The goal of this work was to characterize the tissue distribution and mechanism of transport of nanoparticles beyond skin, with and without Ultraviolet Radiation (UVR) induced skin barrier disruption. Atomic absorption spectroscopy (AAS), flow cytometry and confocal microscopy were used to examine the effect of UVR dose (180 and 360 mJ/cm2 UVB) on the skin penetration and systemic distribution of quantum dot (QD) nanoparticles topically applied at different time-points post UVR using a hairless C57BL/6 mouse model. Results indicate that QDs can penetrate mouse skin, regardless of UVR exposure, as evidenced by the increased cadmium in the local lymph nodes of all QD treated mice. The average % recovery for all treatment groups was 69.68% with ~66.84% of the applied dose recovered from the skin (both epicutaneous and intracutaneous). An average of 0.024% of the applied dose was recovered from the lymph nodes across various treatment groups. When QDs are applied 4 days post UV irradiation, at the peak of the skin barrier defect and LC migration to the local lymph node, there is an increased cellular presence of QD in the lymph node; however, AAS analysis of local lymph nodes display no difference in cadmium levels due to UVR treatment. Our data suggests that Langerhans cells (LCs) can engulf QDs in skin, but transport to the lymph node may occur by both cellular (dendritic and macrophage) and non-cellular mechanisms. It is interesting that these specific nanoparticles were retained in skin similarly regardless of UVR barrier disruption, but the observed skin immune cell interaction with nanoparticles suggest a potential for immunomodulation, which we are currently examining in a murine model of skin allergy. The online version of this article (doi:10.1186/s12989-017-0191-7) contains supplementary material, which is available to authorized users.