Evidence for glycinergic respiratory neurons: Bötzinger neurons express mRNA for glycinergic transporter 2
Evidence for glycinergic respiratory neurons: Bötzinger neurons express mRNA for glycinergic transporter 2
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甘氨酸呼吸神经元的证据：Bötzinger 神经元表达甘氨酸转运蛋白 2 的 mRNA
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
P. Guyenet
中科院分区：
文献类型：
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作者：
A. Schreihofer;R. Stornetta;P. Guyenet
在呼吸周期的呼气后期，延髓吻侧腹外侧的Bötzinger （BÖTZ）神经元放电。这些细胞抑制延髓中膈运动神经元和几种类型的呼吸神经元。BÖTZ细胞对其目标神经元产生快速的氯化物介导的抑制，但这些细胞使用的神经递质尚未确定。在本研究中，我们研究γ -氨基丁酸（GABA）或甘氨酸是否可能是BÖTZ细胞的抑制性神经递质。在氯氯蔗糖麻醉的大鼠中，我们使用细胞旁标记法分别用生物素酰胺填充20个具有生理特征的BÖTZ神经元。含有标记BÖTZ神经元的髓质切片使用地高辛标记的谷氨酸脱羧酶异构体67 (GAD67) （gaba能神经元的标记物）或甘氨酸转运蛋白2 (GLYT2)（甘氨酸能神经元的标记物）的核糖探针进行原位杂交。所有BÖTZ细胞均含有GLYT2 mRNA (n = 10)，而GAD67 mRNA均未检测到（n = 10）。作为阳性对照，记录了12个黑质网状部gaba能神经元，并在体内充满生物素酰胺。正如预期的那样，这些细胞中的大多数具有可检测到的GAD67 mRNA水平（12个细胞中的11个）。这些结果表明，细胞旁标记方法可以与原位杂交相结合，以识别生理特征细胞可能具有gaba能或甘氨酸能表型。此外，这些数据表明BÖTZ神经元使用神经递质甘氨酸而不是GABA来提供对呼吸相关神经元的广泛抑制。[j]中华神经科杂志，2004,27(4):583 - 597。©1999 Wiley‐Liss, Inc。
Bötzinger (BÖTZ) neurons in the rostral ventrolateral medulla fire during the late expiratory phase of the respiratory cycle. These cells inhibit phrenic motor neurons and several types of respiratory neurons in the medulla oblongata. BÖTZ cells produce a fast, chloride‐mediated inhibition of their target neurons, but the neurotransmitter used by these cells has not been determined. In the present study, we examine whether γ‐aminobutyric acid (GABA) or glycine could be the inhibitory neurotransmitter of BÖTZ cells. In chloralose‐anesthetized rats, we individually filled 20 physiologically characterized BÖTZ neurons with biotinamide by using a juxtacellular labeling method. Medullary sections containing the labeled BÖTZ neurons were processed for in situ hybridization by using digoxigenin‐labeled riboprobes for glutamic acid decarboxylase isoform 67 (GAD67), a marker for GABAergic neurons, or for glycine transporter 2 (GLYT2), a marker for glycinergic neurons. All BÖTZ cells examined contained GLYT2 mRNA (n = 10), whereas none had detectable levels of GAD67 mRNA (n = 10). For a positive control, 12 GABAergic neurons in the substantia nigra pars reticulata also were recorded and filled with biotinamide in vivo. Most of these cells, as expected, had detectable levels of GAD67 mRNA (11 out of 12). These results demonstrate that the juxtacellular labeling method can be combined with in situ hybridization to identify physiologically characterized cells with probable GABAergic or glycinergic phenotypes. Furthermore, these data suggest that BÖTZ neurons use the neurotransmitter glycine and not GABA to provide widespread inhibition of respiratory‐related neurons. J. Comp. Neurol. 407:583–597, 1999. © 1999 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.89.15.7189
发表时间：
1992-08-01
影响因子：
11.1
作者：
GUASTELLA, J;BRECHA, N;DAVIDSON, N
通讯作者：
DAVIDSON, N