Pregnancy dating in the rat: Placental morphology and maternal blood parameters
Pregnancy dating in the rat: Placental morphology and maternal blood parameters
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DOI：
10.1080/019262302753559614
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发表时间：
2002-03-01
影响因子：
1.5
通讯作者：
Flik, G
中科院分区：
文献类型：
--
作者：
de Rijk, EPCT;van Esch, E;Flik, G
大鼠是胚胎学和生殖毒理学研究中常用的动物模型。令人惊讶的是，目前的文献没有提供关于大鼠胎盘发育的全面参考数据集。因此，我们评估了孕期SD大鼠胎盘和母体血液参数的形态变化。本研究提供的形态数据可作为参考资料。这项研究揭示了大鼠胎盘发育是一个明确的过程，其特征是特定时间点的关键同步形态事件，便于实验室实践，并为毒理学家提供了区分正常和异常胎盘发育以及检测胎儿和胎盘错配的灵敏工具。在大鼠怀孕期间，观察到母亲血液参数的显著变化，这与在怀孕妇女中观察到的情况非常相似。这些变化包括：(A)血液稀释导致的血细胞体积减少，(B)反映母亲对同种异体胎儿移植反应的白细胞计数增加，(C)凝血值增加，(D)降低血糖和增加脂肪含量，以最大限度地利用胎儿葡萄糖和保护母体能量，(E)反映血浆体积扩大的电解液数值下降。结论：孕鼠胎盘形态和母体血液参数的联合数据集为识别异常妊娠提供了有力的工具。
The rat is commonly used as a model in studies on embryology and reproduction toxicology. Surprisingly, the current literature does not provide a comprehensive reference data set on placental development in rat. Therefore, we have evaluated morphological changes of the placenta and maternal blood parameters during pregnancy of the Sprague-Dawley rat. The morphologic data presented in this study may be useful as reference material. This study revealed that placental development in the rat is a well-defined process, characterized by key synchronized morphological events at specific points in time, convenient for laboratory practice and provides the toxicologist with a sensitive tool to distinguish between normal and abnormal placental development and to detect fetal and placental mismatches. During rat pregnancy, significant changes were observed in maternal blood parameters strongly reminiscent of those observed in pregnant women. These changes included: (a) decreased blood cell volume as a result of hemodilution, (b) increased white blood cell counts reflecting the response of the mother to the fetal allograft, (c) increased blood clotting values, (d) decreased plasma glucose and increased lipid content maximizing fetal glucose availability and maternal energy conservation, and (e) decreased electrolyte values reflecting plasma volume expansion. It was concluded that the combined data set on placental morphology and maternal blood parameters in pregnant rats provides powerful tools for recognition of abnormal pregnancies.