Probing the Deep Brain: Enhanced Multi-Photon Imaging by Aggregation-Induced Emission Luminogens via Nanocrystallization
Probing the Deep Brain: Enhanced Multi-Photon Imaging by Aggregation-Induced Emission Luminogens via Nanocrystallization
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DOI：
10.1016/j.cej.2023.142850
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发表时间：
2023-04
影响因子：
15.1
通讯作者：
Zhourui Xu;Xiangquan Deng;Gang Feng;Wanjian Zhang;Yibin B. Zhang;Wenguang Zhang;Miaozhuang Fan;Mengni Hu;Chengbin Yang;Ming Ying;Yuanyuan Shen;Weixin Xie;Ke Wang;Gaixia Xu
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文献类型：
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作者：
Zhourui Xu;Xiangquan Deng;Gang Feng;Wanjian Zhang;Yibin B. Zhang;Wenguang Zhang;Miaozhuang Fan;Mengni Hu;Chengbin Yang;Ming Ying;Yuanyuan Shen;Weixin Xie;Ke Wang;Gaixia Xu
光学成像的最新要求是以实时和微创模式可视化重要的，厚的和复杂的生物组织。在各种可用的诊断工具中，具有长波长激发和卓越横向分辨率的多光子成像（MPI）一直是一个完美的选择。然而，现有的MPI造影剂的调色板或者遭受次优成像性能或者在生物安全性方面的不可忽略的风险，因此探索合适的探针具有重要意义。为了解决这些问题，在这项工作中，纯有机纳米晶体（NC）开发的基础上聚集诱导发射发光体（AIEgens）（DPTA-BT）与超声辅助的方法。与无定形的DPTA-BT纳米颗粒（NPs）相比，具有有序排列的分子的NCs表现出高度增强的非线性光学信号，包括高达15.35倍的双光子作用截面（2PACS）和高达4.11倍的三光子作用截面（3 PACS）。MPI和DPTA-BT NC的组合有助于开颅术后血管系统的1900 μm成像深度和通过完整颅骨的765 μm成像深度。体外和体内生物安全性评价证实了DPTA-BT NC的安全性。因此，这项工作有望鼓励更多的创新，在MPI的有机造影剂的发展。
The latest requirement of optical imaging is to visualize vital, thick, and complex biological tissues in real-time and minimum-invasive mode. Among diverse available diagnostic tools, multi-photon imaging (MPI) with long wavelength excitation and exceptional lateral resolution has been a perfect option. However, the existing palette of MPI contrast agents either suffers from sub-optimal imaging performance or non-negligible risk in biosafety, exploration of suitable probes is thus of great significance. To address these concerns, in this work, pure organic nanocrystals (NCs) were developed based on aggregation-induced emission luminogens (AIEgens) (DPTA-BT) with an ultrasound-assisted method. Compared to the amorphous DPTA-BT nanoparticles (NPs), NCs with orderly arranged molecules exhibited highly enhanced nonlinear optical signals, including up to 15.35 folds of two-photon action cross-section (2PACS) and up to 4.11 folds of three-photon action cross-section (3PACS). The combination of MPI and DPTA-BT NCs has contributed to a 1900 μm imaging depth of vasculature after craniotomy and a 765 μm imaging depth through the intact skull. The safe use of DPTA-BT NCs was verified by thein vitroandin vivobiosafety assessment. This work is thus expected to encourage more innovation in the development of organic contrast agents for MPI.