Novel system for determining contrast agent concentration in mouse blood in vivo
Novel system for determining contrast agent concentration in mouse blood in vivo
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DOI：
10.1002/mrm.10727
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发表时间：
2004-03-01
影响因子：
3.3
通讯作者：
Bhujwalla, ZM
中科院分区：
文献类型：
--
作者：
Pathak, AP;Artemov, D;Bhujwalla, ZM
开发了一种用于测量血液中造影剂浓度的方法，该方法使用可切换RF尾线圈从小鼠尾静脉获得MR信号。可切换RF线圈用于获得尾部的定量高分辨率T-I图，与第二线圈组合用于从小鼠肿瘤模型中的感兴趣区域获得图像。使用流动体模验证流速对T-1测量的影响。血液T-1的可靠估计获得的流速高达0.5厘米/秒的SCID小鼠。在体内尾线圈测量和体外血液样品的血液松弛率之间没有显著差异。测量小鼠体内血液中造影剂浓度的能力可用于多种应用，例如获得肿瘤血管体积和渗透性的定量值，确定药物递送至肿瘤的有效性，或用于检测淋巴引流。(C)2004 Wiley-Liss，Inc.
A method for measuring contrast agent concentration in blood was developed using a switchable RF tail coil to obtain MR signal from the mouse tail vein. The switchable RF coil was used to obtain quantitative high-resolution T-1 maps of the tail in combination with a second coil used to obtain images from regions of interest in a mouse tumor model. The effect of flow rates on the T-1 measurements was validated using a flow-phantom. Reliable estimates of blood T-1 were obtained for flow rates up to 0.5 cm/sec in SCID mice. There was no significant difference between the in vivo tail coil measurements and in vitro blood sample relaxation rates of blood. The ability to measure the concentration of contrast agents in mouse blood in vivo is useful for several applications, such as obtaining quantitative values of tumor vascular volume and permeability, determining the effectiveness of drug delivery to tumors, or for detecting lymphatic drain. (C) 2004 Wiley-Liss, Inc.