SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells
SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells
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SQLE促进牛骨骼肌间充质干细胞的分化和凋亡
DOI：
10.1089/cell.2019.0077
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发表时间：
2020
影响因子：
1.6
通讯作者：
Sufang Yang
中科院分区：
文献类型：
--
作者：
Ruimen Zhang;Yanfei Deng;Qiao Lv;Qinghua Xing;Yu Pan;Jingyuan Liang;Mingsheng Jiang;Yingming Wei;Deshun Shi;Bingkun Xie;Sufang Yang
本研究将角鲨烯环氧酶（SQLE）过表达载体转染牛骨骼肌间充质干/基质细胞（MSCs），探讨SQLE通过肌肉发生调控肉质的分子机制。我们初步分析了SQLE在牛胚胎和成体、4个不同牛品种的肌肉组织以及广西牛品种的11个不同组织/器官中的表达。随后，分离培养牛骨骼肌源性MSCs，检测其增殖分化过程中SQLE的表达.然后构建牛源性SQLE过表达载体，通过脂质体转染法将其转染牛源性骨骼肌间充质干细胞。细胞计数试剂盒-8（CCK-8）、5-乙炔基-20-脱氧尿苷（EdU）、流式细胞术、免疫荧光和定量聚合酶链反应测定用于详细表征细胞增殖和分化。结果表明，牛SQLE基因在脑组织中的相对表达量最高，在成体肌肉组织中的相对表达量显著高于胚胎期。特别是在细胞分化阶段，SQLE的表达显著上调。增殖、细胞周期、凋亡和成肌细胞分化实验表明，SQLE能显著促进牛骨骼肌源性MSCs的分化和凋亡，但抑制其增殖。总之，我们的研究揭示了SQLE在成肌细胞分化中的作用。这些结果将为牛肌肉发育的调控网络提供新的线索。
In this study, Squalene epoxidase (SQLE) overexpression vector was transfected into bovine skeletal muscle-derived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism ofSQLEregulating meat quality through myogenesis. We initially profiled the expression ofSQLEin cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression ofSQLEduring cell proliferation and differentiation. Then, we constructed a bovineSQLEoverexpression vector and transfected it into bovine skeletal muscle-derived MSCs by liposome transfection. Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), flow cytometry, immunofluorescence, and quantitative polymerase chain reaction assays were used to characterize cell proliferation and differentiation in detail. The results showed that the relative expression level of bovineSQLEgene in brain tissue was the highest, and in adult muscle tissue was significantly higher than that in embryonic stage. Especially, the expression ofSQLEwas significantly upregulated in cell differentiation stage. Furthermore, the proliferation, cell cycle, apoptosis, and myoblast differentiation assays indicated thatSQLEsignificantly promoted the differentiation and apoptosis of bovine skeletal muscle-derived MSCs, but inhibited their proliferation. In conclusion, our study reveals the role ofSQLEin myoblast differentiation. These results will provide new clues for the regulation network of bovine muscle development.