Expression of dentin sialoprotein (DSP) and other molecular determinants by a new cell line from dental papillae, MDPC-23
Expression of dentin sialoprotein (DSP) and other molecular determinants by a new cell line from dental papillae, MDPC-23
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DOI：
10.3109/03008209809002443
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Butler, WT
中科院分区：
文献类型：
--
作者：
Sun, ZL;Fang, DN;Butler, WT
本研究的目的是研究CD-1胎鼠磨牙乳头自发永生化克隆细胞系MDPC-23的分子表达，以确定这些细胞是否为成牙本质细胞样细胞。免疫印迹显示，在第7、37和77代细胞的裂解物、第37代细胞的无血清条件培养液和小鼠牙本质提取液中，均出现一条约105 kDa的蛋白条带，与抗DSP抗体呈阳性反应，并与小鼠DSP共迁移。在细胞裂解产物中也有一条55 kDa的小条带，用486bp的小鼠DSP编码序列的cDNA探针，RT-PCR后的Northern分析和Southern分析都检测到了DSP或DSP-PP的mRNA表达。在这些细胞中，1，25(OH)(2)维生素D(3)上调骨桥蛋白和骨钙素的mRNA，地塞米松下调碱性磷酸酶和α(2)(I)胶原的mRNA，从而使MDPC-23细胞表达矿化组织所共有的蛋白质。DSP和DSP-PP的表达强烈提示该细胞系来源于成牙本质细胞系。
The purpose of this study was to characterize the molecular expression of a spontaneously immortalized and cloned cell line (MDPC-23) derived from 18-19 day CD-1 fetal mouse molar dental papillae to determine if these cells were odontoblast-like. Western blots showed that a protein band, at approximately 105 kDa, reacting positively with anti-DSP antibodies and co-migrating with mouse DSP, was present in lysates of cells from passages 7, 37 and 77, in serum-free conditioned medium from passage 37 cells, and in mouse dentin extract. A minor band at 55 kDa was also apparent in cell lysates, Using a cDNA probe for a 486 bp mouse DSP coding sequence, DSP or DSP-PP mRNA expression was detected by Northern analysis as well as Southern analysis after RT-PCR in all three passages. It was also shown that in these cells 1,25 (OH)(2) vitamin D(3) upregulated both osteopontin and osteocalcin mRNA, and dexamethasone downregulated alkaline phosphatase and alpha(2)(I) collagen mRNA, Thus, MDPC-23 cells express proteins which are common to mineralizing tissue. The expression of DSP and DSP-PP strongly suggests that this cell line is from the odontoblast lineage.