Effects of phosphate glass fiber-collagen scaffolds on functional recovery of completely transected rat spinal cords
Effects of phosphate glass fiber-collagen scaffolds on functional recovery of completely transected rat spinal cords
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DOI：
10.1016/j.actbio.2012.01.026
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发表时间：
2012-05-01
影响因子：
9.7
通讯作者：
Hyun, Jung Keun
中科院分区：
文献类型：
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作者：
Joo, Na-Young;Knowles, Jonathan C.;Hyun, Jung Keun
基于磷酸盐的玻璃纤维（PGF），由于其生物降解性和方向性等特性，可以有效地作为神经损伤后轴突生长的空间线索。在本研究中，开发了直径为1.8 mm、长度为3 mm的含PGF的圆柱形支架，并将其植入T9大鼠脊髓完全横断后近端和远端残端之间的差距中。对照组将不含PGF的胶原支架植入横断脊髓内。旷场Basso、Beattie和Bresnahan运动量表显示，植入后8 - 12周，实验组的运动功能优于对照组，尿动力学分析显示实验组在某些参数上有额外的改善。植入12周后，实验组近端和远端残端有轴突生长到支架上，而对照组无。在植入后1周和4周，巨噬细胞围绕受损的胸脊髓;然而，植入后6小时，促炎细胞因子在对照组和实验组之间没有差异。皮质脊髓束（CST）生物素葡聚糖胺顺行示踪显示，实验组部分CST产物可到达腰膨大处。到12周时，实验组膀胱中脑源性神经营养因子的mRNA水平比对照组增加更多。我们的结论是，PGFs可以有一个有益的影响，功能恢复后完全横断大鼠胸脊髓。(C)2012 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Phosphate-based glass fibers (PGFs), due to characteristics such as biodegradability and directionality, could be effective as spatial cues for axonal outgrowth following nerve injury. In the present study, PGF-containing cylindrical scaffolds of 1.8 mm diameter and 3 mm length were developed and implanted into the gap between the proximal and distal stumps following complete transection of rat spinal cords at T9. The PGF-free collagen scaffolds were implanted into the transected spinal cords of the control group. The open-field Basso, Beattie and Bresnahan locomotor scale revealed that the locomotor function of the experimental group was better than in the control group from 8 to 12 weeks after implantation, and urodynamic analysis revealed additional improvements in the experimental group in some parameters. Twelve weeks after implantation, some axon growth from the proximal and distal stumps to the scaffold was observed in the experimental group but not in the control group. Macrophages surrounded the injured thoracic spinal cord at 1 and 4 weeks after implantation; however, 6 h after implantation, the pro-inflammatory cytokines did not differ between the control and experimental groups. Anterograde corticospinal tract (CST) tracing with biotinylated dextran amine showed that, in the experimental group, some CST outgrowths could reach the lumbar enlargement. By 12 weeks, the mRNA levels of brain-derived neurotrophic factor in the bladder had increased more in the experimental group than in the controls. We conclude that PGFs can have a beneficial effect on functional recovery following complete transection of the thoracic spinal cord in rats. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.