Significance of the expression of the CER6 condensing enzyme for cuticular wax production in Arabidopsis
Significance of the expression of the CER6 condensing enzyme for cuticular wax production in Arabidopsis
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DOI：
10.1104/pp.003707
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发表时间：
2002-08-01
期刊：
影响因子：
7.4
通讯作者：
Kunst, L
中科院分区：
文献类型：
--
作者：
Hooker, TS;Millar, AA;Kunst, L
为了了解更多关于CER 6凝聚酶在拟南芥表面蜡生产中的作用，我们确定了CER 6转录结构域和CER 6转录在幼年、成熟和衰老组织的营养和生殖结构中的时间。我们发现CER 6在整个发育过程中高度转录，仅在检查的所有组织中的表皮细胞中。表皮表达的唯一例外是在接近成熟的花药中观察到的，其中CER 6 mRNA定位于绒毡层中。为了确定是否环境因素，如光和水分亏缺，这是已知的刺激蜡积累，诱导CER 6转录，我们研究了这些因素对CER 6转录丰度的影响。我们的研究结果表明，光是必不可少的CER 6转录，渗透胁迫和脱落酸的存在下，增强CER 6转录积累。CER 6启动子指导的β-葡萄糖醛酸酶报告基因在转基因植物中的表达表明，CER 6启动子在指导拟南芥和烟草（烟草）中的表皮特异性表达中是非常有效的。此外，CER 6启动子驱动的CER 6过表达导致拟南芥茎中蜡沉积增加。这些实验表明，表皮中CER 6的表达水平是控制拟南芥茎上蜡积累的因素之一。
To learn more about the role of the CER6 condensing enzyme in Arabidopsis surface wax production, we determined CER6 transcription domains and the timing of CER6 transcription in vegetative and reproductive structures from juvenile, mature, and senescing tissues. We found that CER6 is highly transcribed throughout development, exclusively in the epidermal cells in all tissues examined. The only exception to the epidermal expression was observed in anthers nearing maturity, in which CER6 mRNA was localized in the tapetum. To determine if environmental factors such as light and water deficit, which are known to stimulate wax accumulation, induce CER6 transcription, we examined the effects of these factors on CER6 transcript abundance. Our results demonstrate that light is essential for CER6 transcription, and that osmotic stress and the presence of abscisic acid enhance CER6 transcript accumulation. CER6 promoter-directed expression of the beta-glucuronidase reporter gene in transgenic plants demonstrated that the CER6 promoter was highly effective in directing epidermis-specific expression in Arabidopsis and tobacco (Nicotiana tabacum). Furthermore, CER6 promoter-driven CER6 overexpression resulted in increased wax deposition in Arabidopsis stems. These experiments indicate that the expression level of CER6 in the epidermis is one of the factors controlling wax accumulation on Arabidopsis stems.