Regulation of prolactin and somatotropin mRNAs by thyroliberin.
Regulation of prolactin and somatotropin mRNAs by thyroliberin.
复制标题
甲状腺激素对催乳素和生长激素 mRNA 的调节。
DOI：
10.1073/pnas.75.3.1294
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发表时间：
1978
影响因子：
11.1
通讯作者：
M. Rosenfeld
中科院分区：
文献类型：
--
作者：
G. Evans;D. David;M. Rosenfeld
三肽促甲状腺素（促甲状腺素释放激素，TRH）调节细胞质催乳素和生长激素（生长激素）mrna的水平在一个功能性大鼠垂体肿瘤细胞。在rna过量杂交反应中，用特异性互补DNA探针测定细胞内mRNA水平，并通过对mRNA定向无细胞翻译产物的二维凝胶电泳分析独立测定细胞内mRNA水平。在培养基中添加TRH后的48小时内，催乳素mRNA序列从含有mRNA的细胞质poly(A)的1.1%增加到4.5%；同时，生长激素mRNA序列减少。在含血清培养基中生长的细胞或在添加TRH之前在无血清培养基中维持72小时的细胞中也观察到催乳素mRNA水平的类似影响，这表明TRH是刺激催乳素mRNA水平的原因，而不是对血清中存在的另一种因子发挥放任作用。
The tripeptide thyroliberin (thyrotropin-releasing hormone, TRH) regulates the levels of cytoplasmic prolactin and growth hormone (somatotropin) mRNAs in a line of functional rat pituitary tumor cells. Intracellular mRNA levels were determined with specific complementary DNA probes in RNA-excess hybridization reactions and independently by two-dimensional gel electrophoretic analysis of the products of mRNA-directed cell-free translation. Prolactin mRNA sequences increased from 1.1 to 4.5% of the cytoplasmic poly(A)containing mRNA within 48 hr after addition of TRH to the medium; in parallel, growth hormone mRNA sequences were decreased. Similar effects on prolactin mRNA levels were observed in cells grown in serum-containing medium or when the cells were maintained in a serum-free medium for 72 hr prior to addition of TRH, suggesting that TRH is responsible for the stimulation of prolactin mRNA levels rather than for exerting a permissive effect for another factor present in serum.