Testing human sera for antibodies against avian influenza viruses: Horse RBC hemagglutination inhibition vs. microneutralization assays
Testing human sera for antibodies against avian influenza viruses: Horse RBC hemagglutination inhibition vs. microneutralization assays
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DOI：
10.1016/j.jcv.2008.04.013
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发表时间：
2008-09-01
影响因子：
8.8
通讯作者：
Gray, Gregory C.
中科院分区：
文献类型：
--
作者：
Kayali, Ghazi;Setterquist, Sharon F.;Gray, Gregory C.
背景：血凝抑制（HI）试验是一种常用的方法来筛选人血清中的甲型流感病毒抗体。由于HI在检测针对禽流感A毒株的抗体中具有相对较差的灵敏度，因此通常优选更复杂的微量中和（MN）测定。最近的研究表明，HI检测的灵敏度可以通过从传统使用的火鸡、豚鼠、人或鸡红细胞切换到马红细胞来提高。目的：评估马红细胞HI在筛选针对H3、H4、H5、H6、H7、H9、H11和H12型禽流感的人抗体时的性能。结果：马红细胞血凝抑制试验具有较高的可靠性结论：马红细胞血凝抑制试验与微核试验结果符合率为52%~ 100%。虽然MN可能仍然是禽流感病毒血清学检测的金标准，但马RBC HI检测可能在大规模流行病学研究中作为筛查检测非常有用。(C)2008年爱思唯尔BN。All rights reserved.
Background: The hemagglutination inhibition (HI) assay is a frequently used method to screen human sera for antibodies against influenza A viruses. Because HI has relatively poor sensitivity in detecting antibodies against avian influenza A strains, a more complicated microneutralization (MN) assay is often preferred. Recent research suggests that the sensitivity of the HI assay can be improved by switching from the traditionally used turkey, guinea pig, human, or chicken RBCs to horse RBCs.Objective: To evaluate the performance of the horse RBC HI when screening for human antibodies against avian influenza types H3, H4, H5, H6, H7, H9, H11, and H12.Study design: We evaluated the reproducibility of horse RBC HI and its agreement with MN results using sera from people exposed or not exposed to wild and domestic birds.Results: The horse RBC HI assay had high reliability (90%-100%) and good agreement with MN assay results (52%-100%).Conclusion: The horse RBC HI assay is reliable, less expensive, less complex, and faster than the MN assay. While MN will likely remain the gold standard serologic assay for avian viruses, the horse RBC HI assay may be very useful as a screening assay in large-scale epidemiologic studies. (C) 2008 Elsevier BN. All rights reserved.