Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model.
Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model.
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DOI：
10.1111/j.1574-695x.2010.00726.x
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发表时间：
2010-11
影响因子：
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通讯作者：
Sadowska-Krowicka H
中科院分区：
文献类型：
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作者：
Bégué RE;Sadowska-Krowicka H
目前正在努力开发一种有效的幽门螺杆菌感染疫苗。我们制备了重组全长（568 aa）幽门螺杆菌尿素酶B蛋白（rUre B），并对其进行了免疫原性和保护性测试。BALB/c小鼠鼻内注射rUreB（40 μg）+CpG（10 μg）、肌肉注射rUreB（50 μg）+3%氢氧化铝（50 μL）或皮下注射rUreB（25 μg）+弗氏佐剂（25 μL），共3次（第0、2和6周）。鼻内rUreB加CpG既无免疫原性也无保护性;肌内rUreB加氢氧化铝具有免疫原性和适度保护性;皮下rUreB加弗氏佐剂具有免疫原性和高度保护性。用弗氏佐剂改善保护的事实表明重组脲酶B是疫苗的良好抗原，但它需要比氢氧化铝更强的佐剂。
Efforts are underway for the development of an effective vaccine against Helicobacter pylori infection. We prepared recombinant full length (568 aa) Helicobacter pylori urease B protein (rUreB) and tested it for immunogenicity and protection. BALB/c mice received either rUreB (40 μg) plus CpG (10 μg) intranasally, rUreB (50 μg) plus 3% aluminum hydroxide (50 μL) intramuscularly or rUreB (25 μg) plus Freund’s adjuvant (25 μL) subcutaneously, three times (week 0, 2 and 6). Intranasal rUreB plus CpG was neither immunogenic nor protective; intramuscular rUreB plus aluminum hydroxide was immunogenic and modestly protective; and subcutaneous rUreB plus Freund’s adjuvant was immunogenic and highly protective. The fact that protection was improved with Freund’s adjuvant indicates that recombinant urease B is a good antigen for a vaccine but that it needs a stronger adjuvant than aluminum hydroxide.