Gene transfer of a putative mammalian K+ channel gene from genomic and cosmid DNA.
Gene transfer of a putative mammalian K+ channel gene from genomic and cosmid DNA.
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来自基因组和粘粒 DNA 的假定哺乳动物 K 通道基因的基因转移。
DOI：
10.1152/ajpcell.1988.255.1.c12
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Gargus,JJ
中科院分区：
文献类型：
--
作者：
Mitas,M;Coogan,C;Gargus,JJ
我们已经成功地转染小鼠基因组DNA含有突变假定的K+通道基因到小鼠LMTK-细胞。掺入突变基因允许原代转化体表达突变细胞表型的增长在阈下K+浓度，而且，导致这些原代转化体显示相同的特征的增长和单向K+流量的突变细胞系，LTK-1，从其中的基因组DNA的来源。从LTK-1突变体DNA形成粘粒基因组文库，并通过转染实验显示含有至少一个独特的粘粒克隆，所述粘粒克隆含有突变体推定的K+通道基因的功能性拷贝。
We have successfully transfected mouse genomic DNA containing a mutant putative K+ channel gene into murine LMTK- cells. Incorporation of the mutant gene allowed primary transformants to express the mutant cell phenotype of growth at a subthreshold K+ concentration and, furthermore, caused these primary transformants to display the same characteristics of growth and unidirectional K+ flux as the mutant cell line, LTK-1, from which the genomic DNA was derived. A cosmid genomic library was formed from LTK-1 mutant DNA and shown by transfection experiments to contain at least one unique cosmid clone containing a functional copy of the mutant putative K+ channel gene.