Physiological responses to central and peripheral injection of polyinosinic-polycytidylic acid in chicks.
Physiological responses to central and peripheral injection of polyinosinic-polycytidylic acid in chicks.
复制标题
雏鸡中枢和外周注射聚肌苷-聚胞苷酸的生理反应。
DOI：
10.1080/00071668.2018.1547361
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发表时间：
2019
影响因子：
2
通讯作者：
Cline MA.
中科院分区：
文献类型：
--
作者：
Tachibana T;Ishimaru Y;Takeda K;Khan MSI;Makino R;Cline MA.
1.本研究的目的是确定侧脑室（ICV）和腹膜内（IP）注射多聚肌苷酸-多聚胞苷酸（poly I：C）（一种结合toll样受体-3（TLR 3）的病毒模拟物）是否影响雏鸡（Gallus gallus）的摄食量、自主活动、泄泻腔温度、血浆皮质酮（CORT）和葡萄糖浓度以及作物排空率.侧脑室和腹腔注射poly I：C均显著降低摄食量. IP而不是ICV注射聚I：C显着抑制自愿活动，而ICV注射减少坐着的时间。侧脑室和腹腔注射poly I：C均显著增加血浆CORT和葡萄糖浓度。侧脑室或腹腔注射poly I：C对泄殖腔温度均无显著影响.另外，侧脑室注射poly I：C可显著降低作物排空率，而腹腔注射则无此作用.这些结果表明，中枢TLR 3与厌食，应激反应和作物排空延迟，而外周TLR 3与厌食，行为变化和应激反应在病毒感染的雏鸡。
1. The purpose of the present study was to determine if intracerebroventricular (ICV) and intraperitoneal (IP) injection of polyinosinic-polycytidylic acid (poly I:C), a viral mimetic that binds to toll-like receptor-3 (TLR3), affects food intake, voluntary activity, cloacal temperature, plasma corticosterone (CORT) and glucose concentrations, and crop emptying rate in chicks (Gallus gallus).2. Both ICV and IP injection of poly I:C significantly decreased food intake.3. IP but not ICV injection of poly I:C significantly suppressed voluntary activity, whereas ICV injection decreased time spent sitting. Both ICV and IP injection of poly I:C significantly increased plasma CORT and glucose concentration. Neither ICV nor IP injection of poly I:C significantly affected cloacal temperature.4. In addition, ICV injection of poly I:C significantly reduced crop emptying rate, whereas IP injection had no effect.5. These results suggested that central TLR3 is related to anorexia, stress response and retardation of crop emptying while peripheral TLR3 is related to anorexia, change in behaviour and stress responses during viral infection in chicks.