Sequence analysis of the HBV S protein in Chinese patients with coexisting HBsAg and anti-HBs antibodies
Sequence analysis of the HBV S protein in Chinese patients with coexisting HBsAg and anti-HBs antibodies
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中国HBsAg和抗HBs抗体共存患者的HBV S蛋白序列分析
DOI：
10.1002/jmv.24273
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发表时间：
2015-12-01
影响因子：
12.7
通讯作者：
Yu, Jie-Ping
中科院分区：
文献类型：
--
作者：
Ding, Feng;Yu, Hong-Gang;Yu, Jie-Ping
B型肝炎病毒（HBV）感染者中存在着B型肝炎表面抗原（HBsAg）和抗HBsAg抗体（抗-HBs），这一现象已被发现并解释了几十年，但仍存在争议。本研究旨在探讨这种特殊的血清学模式与S基因区突变的关系。选择15例HBsAg和抗-HBs同时存在的患者作为实验组，27例单纯HBsAg阳性患者作为对照组。扩增S基因片段并测序。两组患者的年龄、性别、丙氨酸氨基转移酶水平、HBsAg滴度、基因型和HBV DNA水平无显著性差异。两组患者均为B、C基因型HBV感染者。与对照组相比，实验组的N-末端区域和MHR的氨基酸变异性更高，尤其是a决定簇。实验组患者中最常见的变化位于位置s126。HBsAg与抗-HBs共存可能与α决定簇氨基酸突变增多有关。对这种血清学模式的临床意义，包括抗-HBs与HBsAg的结合、免疫逃逸以及抗病毒治疗的疗效等，有待进一步研究。医学病毒学杂志87：2067-2073，2015。(c)2015 Wiley Periodicals，Inc.
The coexistence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in patients with hepatitis B virus (HBV) infection has been discovered and explained for several decades, but debate still exists. This study was to explore the relationship between this special serological pattern and mutations in S gene region. Fifteen patients with coexisting HBsAg and anti-HBs were selected as the experimental group, and 27 patients with HBsAg positive only were selected as the control group. The S gene region was amplified and sequenced. No significant differences were observed between the two groups with regard to age, gender, alanine aminotransferase level, HBsAg titer, genotype, and HBV DNA level. The patients from the two groups were infected with HBV of the genotype B and C. Compared with the control group, the experimental group showed a higher variability in amino acid within the N-terminal region and the MHR, especially the a determinant. The most frequent change in patients from the experimental group was located at positions s126. The coexistence of HBsAg and anti-HBs might be associated with the increased amino acid mutations in the a determinant. Further studies should be performed to determine the clinical implication of this serological pattern, including the binding of anti-HBs to HBsAg, escape from immune system, and efficacy of antiviral therapy. J. Med. Virol. 87:2067-2073, 2015. (c) 2015 Wiley Periodicals, Inc.